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Statements

Subject Item
n2:RIV%2F70565813%3A_____%2F11%3A%230000250%21RIV12-MV0-70565813
rdf:type
skos:Concept n5:Vysledek
dcterms:description
This work is a follow-up to an earlier one in which we presented the radioprotective properties of a watersoluble C60 fullerene derivative (DF) which had been obtained by a reaction of C60 fullerene with peracetic acid and subsequent hydrolysis. Radioprotective properties were proven in vivo (testing organism – danio rerio, the concentration of DF solution 147 mg/l) as were its non-toxic properties on this sensitive organism. But we observed cytotoxic properties in vitro on guinea pig kidney cells (as a testing culture) and largely on melanoma cells. In this work we pursued the reasons for cell toxicity. We used the NanoSight LM10HSB analysis for determining the nanoparticle size and concentration in used DF solution of 147 mg/l. It was confirmed that the system contained larger particles than 100 nm and was polydispersed. After filtration with a 0,1μm filter and under a 20x and 10x objective, the system showed a nice and quite monodispersed distribution of modal particle size 42 + 53 and 60 nm in concentration 3.5x108 particles/ml and 10x108 particles/ml respectively. We can explain the cytotoxic properties in vitro by different particle size in the polydispersed DF solution which was not significant for the entire biological system (danio rerio) living in DF solution, but which was important for the separated different cells culture. During repeated In vitro experiments, the nanoparticles smaller than 60 nm were able to penetrate into the cells and did not cause physical cytotoxicity associated with the coagulation of aggregates on the cell surfaces like as before the filtration. The radioprotective effect of DF in vitro developed as well. The cytotoxicity on melanoma cells was proven in both cases, but the mechanism could have been different. In any case, this is the positive medical effect with a potential utilization in cancer radiotherapy . This work is a follow-up to an earlier one in which we presented the radioprotective properties of a watersoluble C60 fullerene derivative (DF) which had been obtained by a reaction of C60 fullerene with peracetic acid and subsequent hydrolysis. Radioprotective properties were proven in vivo (testing organism – danio rerio, the concentration of DF solution 147 mg/l) as were its non-toxic properties on this sensitive organism. But we observed cytotoxic properties in vitro on guinea pig kidney cells (as a testing culture) and largely on melanoma cells. In this work we pursued the reasons for cell toxicity. We used the NanoSight LM10HSB analysis for determining the nanoparticle size and concentration in used DF solution of 147 mg/l. It was confirmed that the system contained larger particles than 100 nm and was polydispersed. After filtration with a 0,1μm filter and under a 20x and 10x objective, the system showed a nice and quite monodispersed distribution of modal particle size 42 + 53 and 60 nm in concentration 3.5x108 particles/ml and 10x108 particles/ml respectively. We can explain the cytotoxic properties in vitro by different particle size in the polydispersed DF solution which was not significant for the entire biological system (danio rerio) living in DF solution, but which was important for the separated different cells culture. During repeated In vitro experiments, the nanoparticles smaller than 60 nm were able to penetrate into the cells and did not cause physical cytotoxicity associated with the coagulation of aggregates on the cell surfaces like as before the filtration. The radioprotective effect of DF in vitro developed as well. The cytotoxicity on melanoma cells was proven in both cases, but the mechanism could have been different. In any case, this is the positive medical effect with a potential utilization in cancer radiotherapy .
dcterms:title
C60 Fullerene Derivative: Influence of Nanoparticles Size on Toxicity and Radioprotectvity of Water Soluble Fullerene Derivate C60 Fullerene Derivative: Influence of Nanoparticles Size on Toxicity and Radioprotectvity of Water Soluble Fullerene Derivate
skos:prefLabel
C60 Fullerene Derivative: Influence of Nanoparticles Size on Toxicity and Radioprotectvity of Water Soluble Fullerene Derivate C60 Fullerene Derivative: Influence of Nanoparticles Size on Toxicity and Radioprotectvity of Water Soluble Fullerene Derivate
skos:notation
RIV/70565813:_____/11:#0000250!RIV12-MV0-70565813
n5:predkladatel
n6:ico%3A70565813
n3:aktivita
n18:P
n3:aktivity
P(VG20102014049)
n3:dodaniDat
n8:2012
n3:domaciTvurceVysledku
n17:1571699
n3:druhVysledku
n4:D
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
192765
n3:idVysledku
RIV/70565813:_____/11:#0000250
n3:jazykVysledku
n21:eng
n3:klicovaSlova
water soluble fullerene derivative; NanoSight analysis; toxicity in vitro; radioprotective properties
n3:klicoveSlovo
n11:water%20soluble%20fullerene%20derivative n11:NanoSight%20analysis n11:toxicity%20in%20vitro n11:radioprotective%20properties
n3:kontrolniKodProRIV
[3F11350FB5BC]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Brno
n3:nazevZdroje
NANOCON 2011
n3:obor
n13:CF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n14:VG20102014049
n3:rokUplatneniVysledku
n8:2011
n3:tvurceVysledku
Zemanová, Eva Zeman, Karel Klouda, Karel
n3:typAkce
n9:WRD
n3:zahajeniAkce
2011-01-01+01:00
s:numberOfPages
10
n15:hasPublisher
TANGER spol. s r.o.
n10:isbn
978-80-87294-23-9