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Statements

Subject Item
n2:RIV%2F68407700%3A21460%2F14%3A00211801%21RIV15-MSM-21460___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Diamond and diamond-like carbon (DLC) films possess a set of excellent physical and chemical properties which together with a high biocompatibility make them attractive candidates for a number of medical and biotechnological applications. In the current work thin ultrananocrystalline diamond (UNCD) and DLC films were comparatively investigated with respect to cell attachment and proliferation after different surface modifications. The UNCD films were prepared by microwave plasma enhanced chemical vapor deposition, the DLC films by pulsed laser deposition (PLD). The films were comprehensively characterized with respect to their basic properties, e.g. crystallinity, morphology, chemical bonding nature, etc. Afterwards the UNCD and DLC films were modified applying O2 or NH3/N2 plasmas and UV/O3 treatments to alter their surface termination. The surface composition of as-grown and modified samples was studied by X-ray photoelectron spectroscopy (XPS). Furthermore the films were characterized by contact angle measurements with water, formamide, 1-decanol and diiodomethane; from the results obtained the surface energy with its dispersive and polar components was calculated. The adhesion and proliferation of MG63 osteosarcoma cells on the different UNCD and DLC samples were assessed by measurement of the cell attachment efficiency and MTT assays. The determined cell densities were compared and correlated with the surface properties of as-deposited and modified UNCD and DLC films. Diamond and diamond-like carbon (DLC) films possess a set of excellent physical and chemical properties which together with a high biocompatibility make them attractive candidates for a number of medical and biotechnological applications. In the current work thin ultrananocrystalline diamond (UNCD) and DLC films were comparatively investigated with respect to cell attachment and proliferation after different surface modifications. The UNCD films were prepared by microwave plasma enhanced chemical vapor deposition, the DLC films by pulsed laser deposition (PLD). The films were comprehensively characterized with respect to their basic properties, e.g. crystallinity, morphology, chemical bonding nature, etc. Afterwards the UNCD and DLC films were modified applying O2 or NH3/N2 plasmas and UV/O3 treatments to alter their surface termination. The surface composition of as-grown and modified samples was studied by X-ray photoelectron spectroscopy (XPS). Furthermore the films were characterized by contact angle measurements with water, formamide, 1-decanol and diiodomethane; from the results obtained the surface energy with its dispersive and polar components was calculated. The adhesion and proliferation of MG63 osteosarcoma cells on the different UNCD and DLC samples were assessed by measurement of the cell attachment efficiency and MTT assays. The determined cell densities were compared and correlated with the surface properties of as-deposited and modified UNCD and DLC films.
dcterms:title
Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications
skos:prefLabel
Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications Cell adhesion and growth on ultrananocrystalline diamond and diamond-like carbon films after different surface modifications
skos:notation
RIV/68407700:21460/14:00211801!RIV15-MSM-21460___
n3:aktivita
n18:I n18:S n18:P
n3:aktivity
I, P(LD12069), S
n3:cisloPeriodika
April
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n4:1722247 n4:4619048 n4:7895712 n4:8421226
n3:druhVysledku
n17:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
6391
n3:idVysledku
RIV/68407700:21460/14:00211801
n3:jazykVysledku
n19:eng
n3:klicovaSlova
ultrananocrystalline diamond films; diamond-like carbon films; surface modification; direct contact cell tests
n3:klicoveSlovo
n6:surface%20modification n6:ultrananocrystalline%20diamond%20films n6:diamond-like%20carbon%20films n6:direct%20contact%20cell%20tests
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[083D48416B8E]
n3:nazevZdroje
Applied Surface Science
n3:obor
n9:BH
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
11
n3:projekt
n11:LD12069
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
297
n3:tvurceVysledku
Reithmaier, J. P. Ceccone, G. Popov, C. Jelínek, Miroslav Apostolova, M. D. Mikšovský, Jan Voss, A. Kocourek, Tomáš Kulisch, W. Písařík, Petr Kozarova, R.
n3:wos
000331717200014
s:issn
0169-4332
s:numberOfPages
8
n8:doi
10.1016/j.apsusc.2014.01.085
n14:organizacniJednotka
21460