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Statements

Subject Item
n2:RIV%2F68407700%3A21460%2F12%3A00202769%21RIV13-MSM-21460___
rdf:type
skos:Concept n4:Vysledek
dcterms:description
The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence (BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by the genetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shape from point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was favourable. The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence (BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by the genetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shape from point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was favourable.
dcterms:title
Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
skos:prefLabel
Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
skos:notation
RIV/68407700:21460/12:00202769!RIV13-MSM-21460___
n4:predkladatel
n5:orjk%3A21460
n8:aktivita
n18:P
n8:aktivity
P(EE.2.3.20.0092)
n8:dodaniDat
n17:2013
n8:domaciTvurceVysledku
n16:6516637 n16:8124280 n16:3657833
n8:druhVysledku
n9:D
n8:duvernostUdaju
n15:S
n8:entitaPredkladatele
n21:predkladatel
n8:idSjednocenehoVysledku
180957
n8:idVysledku
RIV/68407700:21460/12:00202769
n8:jazykVysledku
n20:eng
n8:klicovaSlova
biosensor; GMO; microbial cell; bioluminiscence
n8:klicoveSlovo
n11:biosensor n11:bioluminiscence n11:microbial%20cell n11:GMO
n8:kontrolniKodProRIV
[E3155198BDE4]
n8:mistoKonaniAkce
Kladno
n8:mistoVydani
Prague
n8:nazevZdroje
Instuments and Methods for Biology and Medicine 2012
n8:obor
n13:JB
n8:pocetDomacichTvurcuVysledku
3
n8:pocetTvurcuVysledku
4
n8:projekt
n19:EE.2.3.20.0092
n8:rokUplatneniVysledku
n17:2012
n8:tvurceVysledku
Jiřina, Marcel Kuncová, G. Kalábová, Hana Pospíšilová, Marie
n8:typAkce
n22:CST
n8:zahajeniAkce
2012-05-29+02:00
s:numberOfPages
3
n10:hasPublisher
České vysoké učení technické v Praze
n3:isbn
978-80-01-05119-1
n7:organizacniJednotka
21460