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Statements

Subject Item
n2:RIV%2F68407700%3A21460%2F12%3A00202752%21RIV13-MSM-21460___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future. The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future.
dcterms:title
Using genetic engineering methods for detection lower fungi in digestive tract of primates Using genetic engineering methods for detection lower fungi in digestive tract of primates
skos:prefLabel
Using genetic engineering methods for detection lower fungi in digestive tract of primates Using genetic engineering methods for detection lower fungi in digestive tract of primates
skos:notation
RIV/68407700:21460/12:00202752!RIV13-MSM-21460___
n10:predkladatel
n11:orjk%3A21460
n3:aktivita
n15:P
n3:aktivity
P(EE.2.3.20.0092)
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n9:8307318 n9:2675595
n3:druhVysledku
n6:D
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
176503
n3:idVysledku
RIV/68407700:21460/12:00202752
n3:jazykVysledku
n5:eng
n3:klicovaSlova
eukaryotic organisms-fungi-in; digestive tract; primates; genetic engineering; , DNA; , PCR condition
n3:klicoveSlovo
n13:eukaryotic%20organisms-fungi-in n13:%20 n13:digestive%20tract n13:genetic%20engineering n13:primates n13:DNA n13:PCR%20condition
n3:kontrolniKodProRIV
[B63A5D383CB4]
n3:mistoKonaniAkce
Kladno
n3:mistoVydani
Prague
n3:nazevZdroje
Instuments and Methods for Biology and Medicine 2012
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n21:EE.2.3.20.0092
n3:rokUplatneniVysledku
n4:2012
n3:tvurceVysledku
Mrázek, J. Jarošíková, Taťána Fliegerová, K. Hamrová, Jitka
n3:typAkce
n19:CST
n3:zahajeniAkce
2012-05-29+02:00
s:numberOfPages
3
n22:hasPublisher
České vysoké učení technické v Praze
n16:isbn
978-80-01-05119-1
n14:organizacniJednotka
21460