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Statements

Subject Item
n2:RIV%2F68407700%3A21460%2F11%3A00192484%21RIV12-MSM-21460___
rdf:type
n11:Vysledek skos:Concept
rdfs:seeAlso
http://imbm.fbmi.cvut.cz/soubory/sbornik.pdf
dcterms:description
The aim of work was detection of micro-eukaryota in faecal samples from healthy people and people suffering from celiac disease and comparison of the results. DNA was isolated from faecal samples of 20 people. It was analyzed using the PCR-DGGE method focused on 18S rRNA genes and PCR focused on fungal ITS regions, both followed by sequencing, and it was tested for presence of 4 bacterial genera (Aspergillus, Candida, Saccharomyces and Penicillium) using real-time PCR. Sequences were compared with database of nucleotide sequences for generic designation. DGGE profiles showed high diversity and low inter individual similarity of the intestinal micro-eukaryota. The sequence analysis revealed presence of Blastocystis sp., Candida albicans and Malassezia restricta, each in one subject. Most of the sequences were similar to different nonspecified sequences from human gut metagenome DNA. Real time PCR detected Saccharomyces cerevisiae in five subjects. No significant differences were found between microflora of healthy and celiac people. The aim of work was detection of micro-eukaryota in faecal samples from healthy people and people suffering from celiac disease and comparison of the results. DNA was isolated from faecal samples of 20 people. It was analyzed using the PCR-DGGE method focused on 18S rRNA genes and PCR focused on fungal ITS regions, both followed by sequencing, and it was tested for presence of 4 bacterial genera (Aspergillus, Candida, Saccharomyces and Penicillium) using real-time PCR. Sequences were compared with database of nucleotide sequences for generic designation. DGGE profiles showed high diversity and low inter individual similarity of the intestinal micro-eukaryota. The sequence analysis revealed presence of Blastocystis sp., Candida albicans and Malassezia restricta, each in one subject. Most of the sequences were similar to different nonspecified sequences from human gut metagenome DNA. Real time PCR detected Saccharomyces cerevisiae in five subjects. No significant differences were found between microflora of healthy and celiac people.
dcterms:title
Eukaryots in human digestive tract Eukaryots in human digestive tract
skos:prefLabel
Eukaryots in human digestive tract Eukaryots in human digestive tract
skos:notation
RIV/68407700:21460/11:00192484!RIV12-MSM-21460___
n11:predkladatel
n15:orjk%3A21460
n3:aktivita
n6:P
n3:aktivity
P(EE.2.3.20.0092)
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n12:8307318
n3:druhVysledku
n8:O
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
198140
n3:idVysledku
RIV/68407700:21460/11:00192484
n3:jazykVysledku
n7:eng
n3:klicovaSlova
gut microflora, micro-eukaryota, yeasts, PCR, DGGE, 18S rRNA, ITS region, celiac disease
n3:klicoveSlovo
n4:yeasts n4:gut%20microflora n4:PCR n4:18S%20rRNA n4:ITS%20region n4:DGGE n4:micro-eukaryota n4:celiac%20disease
n3:kontrolniKodProRIV
[477C5DC7F9C9]
n3:obor
n17:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n10:EE.2.3.20.0092
n3:rokUplatneniVysledku
n5:2011
n3:tvurceVysledku
Valtrová, T. Mrázek, J. Fliegerová, K. Jarošíková, Taťána
n18:organizacniJednotka
21460