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Statements

Subject Item
n2:RIV%2F68407700%3A21460%2F08%3A12145530%21RIV09-MSM-21460___
rdf:type
skos:Concept n19:Vysledek
dcterms:description
Pro dosažení objemové rekonstrukce elasticky registrujeme konfokální obrazy z následných fyzických řezů. Využitím obrazů z USB světelného mikroskopu se přesnost registrace zvýší. Postup je následující: Nejdříve se registrují konfokální data vůči obrazům z USB mikroskopu a následně se registrují již přizpůsobená konfokální data vůči sobě. Výše popsaným způsobem dosahujeme dvojího cíle. Jednak kvantifikujeme míru deformací a tím chybu rekonstrukce a jednak na základě znalosti této chyby docilujeme přesnější objemové rekonstrukce. We reconstruction results using a 17-day-old embryo of a laboratory Norway rat here. Ten confocal images of physical slices of the embryo were aligned with corresponding images of these slices captured by the USB light microscope by elastic registration. We measured areas of embryo slices from confocal data both before and after alignment and also from USB light microscope data. The measurements revealed partial deformations of cut physical slices (decreasing / increasing of areas measured from original confocal data with respect to USB light microscope data up to 92.7% / 105.3%), but an extensive one as well (decreasing the area of the embryo slice in confocal data to 55% only). However, even this dramatic deformation was successfully compensated by elastic alignment. Thus, our method for compensation of deformations using accessorial data from the USB light microscope turns up to be efficient and providing us with realistic visualization of biological structures under study. We reconstruction results using a 17-day-old embryo of a laboratory Norway rat here. Ten confocal images of physical slices of the embryo were aligned with corresponding images of these slices captured by the USB light microscope by elastic registration. We measured areas of embryo slices from confocal data both before and after alignment and also from USB light microscope data. The measurements revealed partial deformations of cut physical slices (decreasing / increasing of areas measured from original confocal data with respect to USB light microscope data up to 92.7% / 105.3%), but an extensive one as well (decreasing the area of the embryo slice in confocal data to 55% only). However, even this dramatic deformation was successfully compensated by elastic alignment. Thus, our method for compensation of deformations using accessorial data from the USB light microscope turns up to be efficient and providing us with realistic visualization of biological structures under study.
dcterms:title
Kompenzace a ohodnocení chyb 3D rekonstrukce z konfokálních mikroskopických obrazů Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images
skos:prefLabel
Kompenzace a ohodnocení chyb 3D rekonstrukce z konfokálních mikroskopických obrazů Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images Compensation and Evaluation of Errors of 3D Reconstructions from Confocal Microscopic Images
skos:notation
RIV/68407700:21460/08:12145530!RIV09-MSM-21460___
n5:aktivita
n13:Z n13:P
n5:aktivity
P(GA102/08/0691), P(IAA100110502), P(IAA500200510), P(LC06063), Z(AV0Z50110509), Z(MSM6840770012)
n5:dodaniDat
n12:2009
n5:domaciTvurceVysledku
n21:3658023
n5:druhVysledku
n18:D
n5:duvernostUdaju
n10:S
n5:entitaPredkladatele
n15:predkladatel
n5:idSjednocenehoVysledku
360726
n5:idVysledku
RIV/68407700:21460/08:12145530
n5:jazykVysledku
n16:eng
n5:klicovaSlova
3D reconstruction; confocal microscopy; error
n5:klicoveSlovo
n6:3D%20reconstruction n6:error n6:confocal%20microscopy
n5:kontrolniKodProRIV
[08D5727C767B]
n5:mistoKonaniAkce
Aachen
n5:mistoVydani
Heidelberg
n5:nazevZdroje
EMC 2008 - 14th European Microscopy Congress
n5:obor
n14:JD
n5:pocetDomacichTvurcuVysledku
1
n5:pocetTvurcuVysledku
6
n5:projekt
n8:LC06063 n8:IAA100110502 n8:GA102%2F08%2F0691 n8:IAA500200510
n5:rokUplatneniVysledku
n12:2008
n5:tvurceVysledku
Brůža, P. Janáček, J. Kocandová, L. Čapek, Martin Kubínová, L. Vagnerová, R.
n5:typAkce
n17:WRD
n5:zahajeniAkce
2008-09-01+02:00
n5:zamer
n11:AV0Z50110509 n11:MSM6840770012
s:numberOfPages
2
n22:hasPublisher
Springer-Verlag
n20:isbn
978-3-540-85154-7
n3:organizacniJednotka
21460