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Statements

Subject Item
n2:RIV%2F68407700%3A21230%2F12%3A00201251%21RIV13-MSM-21230___
rdf:type
skos:Concept n4:Vysledek
dcterms:description
Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluating the results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allows in comparison with commercial software faster and easier evaluation of the electrophoreogram images mainly due to segmentation of images. Gel electrophoresis is one of the most frequently used methods in life-sciences research. Electrophoresis is a chemical separation method based on the effect of electric field on the particles. In this paper we present our proposed method for evaluating the results of gel electrophoresis images - electrophoreogram. The evaluation of the electrophoreogram images, i.e. to determine the molecular weight of divided particles, is commonly used commercial software. Our method segments image, automatically extracts the brightness curves across individual lanes, finds divided substances (bands) in these curves and calculates molecular weight of divided substances. We implemented the method and verified its success in comparison with commercial software on electrophoreogram images acquired using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). We found that the proposed method gives reliable results comparable with the evaluation using commercial software. Our method allows in comparison with commercial software faster and easier evaluation of the electrophoreogram images mainly due to segmentation of images.
dcterms:title
Processing Electrophoreogram Image Processing Electrophoreogram Image
skos:prefLabel
Processing Electrophoreogram Image Processing Electrophoreogram Image
skos:notation
RIV/68407700:21230/12:00201251!RIV13-MSM-21230___
n4:predkladatel
n5:orjk%3A21230
n6:aktivita
n11:Z
n6:aktivity
Z(MSM6840770038)
n6:dodaniDat
n7:2013
n6:domaciTvurceVysledku
n21:9942904 n21:3799158 n21:5112605
n6:druhVysledku
n22:D
n6:duvernostUdaju
n10:S
n6:entitaPredkladatele
n18:predkladatel
n6:idSjednocenehoVysledku
162408
n6:idVysledku
RIV/68407700:21230/12:00201251
n6:jazykVysledku
n19:eng
n6:klicovaSlova
Gel Electrophoresis; Evaluation; Signal Processing
n6:klicoveSlovo
n15:Evaluation n15:Signal%20Processing n15:Gel%20Electrophoresis
n6:kontrolniKodProRIV
[EDA0C2CA7B87]
n6:mistoKonaniAkce
Praha
n6:mistoVydani
Praha
n6:nazevZdroje
BioDat 2012 - Conference on Advanced Methods of Biological Data and Signal Processing
n6:obor
n12:EI
n6:pocetDomacichTvurcuVysledku
3
n6:pocetTvurcuVysledku
7
n6:rokUplatneniVysledku
n7:2012
n6:tvurceVysledku
Vysloužilová, Lenka Anýž, Jiří Štěpánková, Olga Křížková, S. Adam, V. Kizek, R. Matijescukova, N.
n6:typAkce
n17:EUR
n6:zahajeniAkce
2012-11-27+01:00
n6:zamer
n9:MSM6840770038
s:numberOfPages
4
n16:hasPublisher
České vysoké učení technické v Praze
n3:isbn
978-80-01-05153-5
n13:organizacniJednotka
21230