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Statements

Subject Item
n2:RIV%2F68378271%3A_____%2F14%3A00434611%21RIV15-GA0-68378271
rdf:type
n16:Vysledek skos:Concept
rdfs:seeAlso
http://scitation.aip.org/content/avs/journal/bip/9/3/10.1116/1.4890471
dcterms:description
The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion. The authors show that nanocrystalline diamond (NCD) thin films prepared by microwave plasma enhanced chemical vapor deposition apparatus with a linear antenna delivery system are well compatible with epithelial cells (5637 human bladder carcinoma) and significantly improve the cell adhesion compared to reference glass substrates. This is attributed to better adhesion of adsorbed layers to diamond as observed by atomic force microscopy (AFM) beneath the cells. Moreover, the cell morphology can be adjusted by appropriate surface treatment of diamond by using hydrogen and oxygen plasma. Cell bodies, cytoplasmic rims, and filopodia were characterized by Peakforce AFM. Oxidized NCD films perform better than other substrates under all conditions (96% of cells adhered well). A thin adsorbed layer formed from culture medium and supplemented with fetal bovine serum (FBS) covered the diamond surface and played an important role in the cell adhesion.
dcterms:title
Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes
skos:prefLabel
Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes Epithelial cells morphology and adhesion on diamonds films deposited and chemically modified by plasma processes
skos:notation
RIV/68378271:_____/14:00434611!RIV15-GA0-68378271
n3:aktivita
n13:I n13:P
n3:aktivity
I, P(GAP108/12/0996), P(KAN400100701)
n3:cisloPeriodika
3
n3:dodaniDat
n17:2015
n3:domaciTvurceVysledku
Ukraintsev, Egor n4:2899841 n4:9994602 Taylor, Andrew n4:5225450
n3:druhVysledku
n12:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
14871
n3:idVysledku
RIV/68378271:_____/14:00434611
n3:jazykVysledku
n8:eng
n3:klicovaSlova
nanocrystalline diamond (NCD); AFM; surfaces; DNA
n3:klicoveSlovo
n5:surfaces n5:nanocrystalline%20diamond%20%28NCD%29 n5:AFM n5:DNA
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[0B775D2612AF]
n3:nazevZdroje
Biointerphases
n3:obor
n14:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n15:KAN400100701 n15:GAP108%2F12%2F0996
n3:rokUplatneniVysledku
n17:2014
n3:svazekPeriodika
9
n3:tvurceVysledku
Ukraintsev, Egor Taylor, Andrew Rezek, Bohuslav Mandys, V. Fendrych, František Krátká, Marie
n3:wos
000340893400013
s:issn
1934-8630
s:numberOfPages
8
n11:doi
10.1116/1.4890471