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Statements

Subject Item
n2:RIV%2F68378271%3A_____%2F12%3A00385037%21RIV13-AV0-68378271
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Boron-doped diamond (BDD) interfaces were chemically functionalized through the catalyst free thiol-yne reaction. Different thiolated precursors (e.g., perfluorodecanethiol, 6-(ferrocenyl)-hexanethiol, DNA) were successfully “clicked to alkynyl-terminated BDD by irradiating the interface at 365 nm for 30 min. Thiolated oligonucleotide strands were immobilized using the optimized reaction conditions, and the surface concentration was tuned to obtain a surface coverage of 3.1 1012 molecules cm–2. Electrochemical impedance spectroscopy (EIS) was employed to follow the kinetics of hybridization and dehybridization events. The sensitivity of the oligonucleotide modified BDD interface was assayed, and a detection limit of 1 nM was obtained. Boron-doped diamond (BDD) interfaces were chemically functionalized through the catalyst free thiol-yne reaction. Different thiolated precursors (e.g., perfluorodecanethiol, 6-(ferrocenyl)-hexanethiol, DNA) were successfully “clicked to alkynyl-terminated BDD by irradiating the interface at 365 nm for 30 min. Thiolated oligonucleotide strands were immobilized using the optimized reaction conditions, and the surface concentration was tuned to obtain a surface coverage of 3.1 1012 molecules cm–2. Electrochemical impedance spectroscopy (EIS) was employed to follow the kinetics of hybridization and dehybridization events. The sensitivity of the oligonucleotide modified BDD interface was assayed, and a detection limit of 1 nM was obtained.
dcterms:title
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
skos:prefLabel
Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events Thiol-yne reaction on boron-doped diamond electrodes: application for the electrochemical detection of DNA-DNA hybridization events
skos:notation
RIV/68378271:_____/12:00385037!RIV13-AV0-68378271
n10:predkladatel
n20:ico%3A68378271
n3:aktivita
n16:P n16:Z
n3:aktivity
P(KAN400100701), Z(AV0Z10100521)
n3:cisloPeriodika
1
n3:dodaniDat
n15:2013
n3:domaciTvurceVysledku
n4:7465416 n4:7664893
n3:druhVysledku
n12:J
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
174355
n3:idVysledku
RIV/68378271:_____/12:00385037
n3:jazykVysledku
n18:eng
n3:klicovaSlova
boron doped diamond; electrochemistry; surface functionalization
n3:klicoveSlovo
n13:surface%20functionalization n13:boron%20doped%20diamond n13:electrochemistry
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[85B129602AA4]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n6:BM
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n17:KAN400100701
n3:rokUplatneniVysledku
n15:2012
n3:svazekPeriodika
84
n3:tvurceVysledku
Kromka, Alexander Houdková, Jana Boukherroub, R. Barras, A. Meziane, D. Szunerits, S.
n3:wos
000298763900030
n3:zamer
n8:AV0Z10100521
s:issn
0003-2700
s:numberOfPages
7
n14:doi
10.1021/ac202350c