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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F98%3A23000086%21RIV%2F2003%2FAV0%2FA23003%2FN
rdf:type
n3:Vysledek skos:Concept
dcterms:description
When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake. When studying non-transferrin-bound iron (NTBI) uptake by mammalian cells in the high-affinity range (micromolar and below), employment of strong chelators such as NTA can lead to kinetic artifacts due to inhibition by excess chelator. This can be avoided by using citrate at an excess concentration above as a little as1,5-fold over iron without danger of precipitation if balanced culture media are used as incubation mediumfor NTBI uptake.
dcterms:title
Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake.
skos:prefLabel
Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake. Pitfalls in Assessing Specific and Affinity of Non-Transferrin-Bound from Uptake.
skos:notation
RIV/68378050:_____/98:23000086!RIV/2003/AV0/A23003/N
n6:strany
128;130
n6:aktivita
n8:Z
n6:aktivity
Z(AV0Z5052915)
n6:cisloPeriodika
1
n6:dodaniDat
n9:2003
n6:domaciTvurceVysledku
n12:3162524 n12:1758330
n6:druhVysledku
n16:J
n6:duvernostUdaju
n7:S
n6:entitaPredkladatele
n14:predkladatel
n6:idSjednocenehoVysledku
778497
n6:idVysledku
RIV/68378050:_____/98:23000086
n6:jazykVysledku
n13:eng
n6:klicovaSlova
N/A
n6:klicoveSlovo
n15:N%2FA
n6:kodStatuVydavatele
US - Spojené státy americké
n6:kontrolniKodProRIV
[E57AB0549305]
n6:nazevZdroje
Analytical Biochemistry
n6:obor
n11:EB
n6:pocetDomacichTvurcuVysledku
2
n6:pocetTvurcuVysledku
4
n6:pocetUcastnikuAkce
0
n6:pocetZahranicnichUcastnikuAkce
0
n6:rokUplatneniVysledku
n9:1998
n6:svazekPeriodika
261
n6:tvurceVysledku
Kriegerbecková, Karin Brunner-Dopper, L. Goldenberg, H. Kovář, Jan
n6:zamer
n17:AV0Z5052915
s:issn
0003-2697
s:numberOfPages
3