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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F14%3A00434353%21RIV15-GA0-68378050
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at 80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples. Microtubules, polymers of the heterodimeric protein αβ-tubulin, are indispensable for many cellular activities such as maintenance of cell shape, division, migration, and ordered vesicle transport. In vitro assays to study microtubule functions and their regulation by associated proteins require the availability of assembly-competent purified tubulin. However, tubulin is a thermolabile protein that rapidly converts into non-polymerizing state. For this reason it is usually stored at 80 °C to preserve its conformation and polymerization properties. In this chapter we describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling evaluation of tubulin functions in rehydrated samples.
dcterms:title
Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin
skos:prefLabel
Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin Stabilization of protein by freeze-drying in the presence of trehalose: a case study of tubulin
skos:notation
RIV/68378050:_____/14:00434353!RIV15-GA0-68378050
n3:aktivita
n13:I n13:P
n3:aktivity
I, P(GAP302/10/1701), P(GPP302/11/P709), P(LH12050)
n3:cisloPeriodika
February
n3:dodaniDat
n8:2015
n3:domaciTvurceVysledku
n6:9103813 n6:7054777 n6:5050383 n6:3093360
n3:druhVysledku
n17:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
46988
n3:idVysledku
RIV/68378050:_____/14:00434353
n3:jazykVysledku
n16:eng
n3:klicovaSlova
Freeze-drying; Microtubules; Stability; Trehalose; Tubulin
n3:klicoveSlovo
n4:Freeze-drying n4:Tubulin n4:Microtubules n4:Trehalose n4:Stability
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[4724B36253F9]
n3:nazevZdroje
Methods in Molecular Biology
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n10:GAP302%2F10%2F1701 n10:GPP302%2F11%2FP709 n10:LH12050
n3:rokUplatneniVysledku
n8:2014
n3:svazekPeriodika
1129
n3:tvurceVysledku
Dráber, Pavel Dráberová, Eduarda Sulimenko, Vadym Sulimenko, Tetyana
n3:wos
000337104300033
s:issn
1064-3745
s:numberOfPages
16
n7:doi
10.1007/978-1-62703-977-2_32