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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F14%3A00431748%21RIV15-GA0-68378050
rdf:type
skos:Concept n20:Vysledek
dcterms:description
The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80°C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies. The chapter 32 covers an elegant method for lyphilization of tubulin. Authors describe step by step the protocol for preparation of freeze-drying (lyophilizated) sample of tubulin. Tubulin is heterodimeric protein that composes microtubules as part of cytoskeleton of the cell. However, tubulin is a thermolabile protein that rapidly converts into non-polymerazing state. For this reason it is usually stored at -80°C to preserve its conformation and polymerization properties. In this chapter authors describe a method for freeze-drying of assembly-competent tubulin in the presence of nonreducing sugar trehalose and methods enabling the evaluation of tubulin functions in rehydrated samples. The chapter also includes images documenting the effect of trehalose on the functionality of the rehydrated tubulin suitable for in vitro studies.
dcterms:title
Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
skos:prefLabel
Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin
skos:notation
RIV/68378050:_____/14:00431748!RIV15-GA0-68378050
n4:aktivita
n13:I n13:P
n4:aktivity
I, P(GAP302/10/1701), P(GAP302/12/1673), P(GPP302/11/P709), P(LH12050)
n4:dodaniDat
n5:2015
n4:domaciTvurceVysledku
n9:7054777 n9:5050383 n9:3093360 n9:9103813
n4:druhVysledku
n18:C
n4:duvernostUdaju
n12:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
46989
n4:idVysledku
RIV/68378050:_____/14:00431748
n4:jazykVysledku
n16:eng
n4:klicovaSlova
Freeze-drying; Microtubules; Stability; Trehalose; Tubulin
n4:klicoveSlovo
n8:Tubulin n8:Microtubules n8:Trehalose n8:Stability n8:Freeze-drying
n4:kontrolniKodProRIV
[699FB0FAF0FB]
n4:mistoVydani
New York
n4:nazevZdroje
Protein Downstream Processing
n4:obor
n15:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetStranKnihy
555
n4:pocetTvurcuVysledku
4
n4:projekt
n11:GAP302%2F10%2F1701 n11:GPP302%2F11%2FP709 n11:GAP302%2F12%2F1673 n11:LH12050
n4:rokUplatneniVysledku
n5:2014
n4:tvurceVysledku
Sulimenko, Vadym Sulimenko, Tetyana Dráber, Pavel Dráberová, Eduarda
s:numberOfPages
16
n19:doi
10.1007/978-1-62703-977-2_32
n14:hasPublisher
Humana Press INC
n7:isbn
978-1-62703-976-5