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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F13%3A00423294%21RIV14-AV0-68378050
rdf:type
skos:Concept n9:Vysledek
dcterms:description
RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes. RNA interference (RNAi) is an evolutionary conserved gene-silencing pathway that can be efficiently utilized as a tool to study gene function. RNAi is initiated by long double-stranded RNAs (dsRNAs), which are processed into small duplexes called small-interfering RNAs (siRNAs). In turn, these duplexes target mRNAs for degradation in a sequence-specific manner. Mouse oocytes, unlike most mammalian cell types, lack an interferon response to long dsRNA. Moreover, they are a rare example of a mammalian cell type with a robust endogenous RNAi pathway. For these reasons microinjection of either long dsRNAs or siRNAs results in efficient, sequence-specific gene silencing. Here, we describe a protocol for preparation and microinjection of long dsRNA into mouse oocytes.
dcterms:title
RNAi-based methods for gene silencing in mouse oocytes RNAi-based methods for gene silencing in mouse oocytes
skos:prefLabel
RNAi-based methods for gene silencing in mouse oocytes RNAi-based methods for gene silencing in mouse oocytes
skos:notation
RIV/68378050:_____/13:00423294!RIV14-AV0-68378050
n9:predkladatel
n10:ico%3A68378050
n3:aktivita
n11:I
n3:aktivity
I
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n15:8484651
n3:druhVysledku
n6:C
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
103126
n3:idVysledku
RIV/68378050:_____/13:00423294
n3:jazykVysledku
n20:eng
n3:klicovaSlova
RNAi; dsRNA; mouse oocyte; microinjection; knockdown
n3:klicoveSlovo
n12:microinjection n12:dsRNA n12:knockdown n12:RNAi n12:mouse%20oocyte
n3:kontrolniKodProRIV
[CED3A720B77E]
n3:mistoVydani
New York
n3:nazevEdiceCisloSvazku
Methods in Molecular Biology, 957
n3:nazevZdroje
Mammalian Oocyte Regulation
n3:obor
n18:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetStranKnihy
311
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n7:2013
n3:tvurceVysledku
Stein, P. Schultz, R. M. Svoboda, Petr
s:numberOfPages
17
n13:doi
10.1007/978-1-62703-191-2
n16:hasPublisher
Humana Press
n8:isbn
978-1-62703-190-5