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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F13%3A00423177%21RIV14-GA0-68378050
rdf:type
skos:Concept n13:Vysledek
dcterms:description
During mouse eye development, all retinal cell types are generated from the population of retina-committed progenitors originating from the neuroepithelium of the optic vesicle. Conditional gene inactivation provides an efficient tool for studying the genetic basis of the developing retina; however, the number of retina-specific Cre lines is limited. Here we report generation of the mRx-Cre BAC transgenic mouse line in which the expression of Cre recombinase is controlled by regulatory sequences of the mouse Rx gene, one of the earliest determinants of retinal development. When mRx-Cre transgenic mice were crossbred with the ROSA26R or ROSA26R-EYFP reporter lines, the Cre activity was observed in the optic sulcus from embryonic day 8.5 onwards and later in all progenitors residing in the neuroepithelium of the optic cup. Our results suggest that mRx-Cre provides a unique tool for functional genetic studies in very early stages of retinal development. Moreover, since eye organogenesis is dependent on the inductive signals between the optic vesicle and head surface ectoderm, the inductive ability of the optic vesicle can be analyzed using mRx-Cre transgenic mice. During mouse eye development, all retinal cell types are generated from the population of retina-committed progenitors originating from the neuroepithelium of the optic vesicle. Conditional gene inactivation provides an efficient tool for studying the genetic basis of the developing retina; however, the number of retina-specific Cre lines is limited. Here we report generation of the mRx-Cre BAC transgenic mouse line in which the expression of Cre recombinase is controlled by regulatory sequences of the mouse Rx gene, one of the earliest determinants of retinal development. When mRx-Cre transgenic mice were crossbred with the ROSA26R or ROSA26R-EYFP reporter lines, the Cre activity was observed in the optic sulcus from embryonic day 8.5 onwards and later in all progenitors residing in the neuroepithelium of the optic cup. Our results suggest that mRx-Cre provides a unique tool for functional genetic studies in very early stages of retinal development. Moreover, since eye organogenesis is dependent on the inductive signals between the optic vesicle and head surface ectoderm, the inductive ability of the optic vesicle can be analyzed using mRx-Cre transgenic mice.
dcterms:title
Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors
skos:prefLabel
Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors Generation of mRx-Cre Transgenic Mouse Line for Efficient Conditional Gene Deletion in Early Retinal Progenitors
skos:notation
RIV/68378050:_____/13:00423177!RIV14-GA0-68378050
n13:predkladatel
n19:ico%3A68378050
n3:aktivita
n15:S n15:P n15:I
n3:aktivity
I, P(ED1.1.00/02.0109), P(GAP305/10/2143), P(GAP305/11/2198), P(LK11214), P(LM2011032), S
n3:cisloPeriodika
5
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n9:5782694 n9:7032919 n9:6641946 n9:7189346 n9:4420403
n3:druhVysledku
n10:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
76341
n3:idVysledku
RIV/68378050:_____/13:00423177
n3:jazykVysledku
n18:eng
n3:klicovaSlova
eye development; lens; retina; transgenic mice
n3:klicoveSlovo
n7:retina n7:lens n7:transgenic%20mice n7:eye%20development
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[E757ED620946]
n3:nazevZdroje
PLoS ONE
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n11:LK11214 n11:GAP305%2F11%2F2198 n11:LM2011032 n11:ED1.1.00%2F02.0109 n11:GAP305%2F10%2F2143
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
8
n3:tvurceVysledku
Machoň, Ondřej Klímová, Lucie Sedláček, Radislav Kozmik, Zbyněk Láchová, Jitka
n3:wos
000319654700099
s:issn
1932-6203
s:numberOfPages
9
n12:doi
10.1371/journal.pone.0063029