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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F12%3A00392142%21RIV14-GA0-68378050
rdf:type
n18:Vysledek skos:Concept
dcterms:description
Limbal stem cells (LSC), which reside in the basal layer of the limbus, are thought to be responsible for corneal epithelial healing after injury. When the cornea is damaged, LSC start to proliferate, differentiate, and migrate to the site of injury. To characterize the signaling molecules ensuring communication between the cornea and LSC, we established a mouse model of mechanical corneal damage. The central cornea or limbal tissue was excised at different time intervals after injury, and the expression of genes in the explants was determined. It was observed that a number of genes for growth and differentiation factors were significantly upregulated in the cornea rapidly after injury. The ability of these factors to regulate the differentiation and proliferation of limbal cells was tested. It was found that the insulin-like growth factor-I (IGF-I), which is rapidly overexpressed after injury, enhances the expression of IGF receptor in limbal cells and induces the differentiation of LSC into cells expressing the corneal cell marker, cytokeratin K12, without any effect on limbal cell proliferation. In contrast, the epidermal growth factor (EGF) and fibroblast growth factor-beta (FGF-beta), which are also produced by the damaged corneal epithelium, supported limbal cell proliferation without any effect on their differentiation. Other factors did not affect limbal cell differentiation or proliferation. Thus, IGF-I was identified as the main factor stimulating the expression of IGF receptors in limbal cells and inducing the differentiation of LSC into cells expressing corneal epithelial cell markers. The proliferation of these cells was supported by EGF and FGF. Limbal stem cells (LSC), which reside in the basal layer of the limbus, are thought to be responsible for corneal epithelial healing after injury. When the cornea is damaged, LSC start to proliferate, differentiate, and migrate to the site of injury. To characterize the signaling molecules ensuring communication between the cornea and LSC, we established a mouse model of mechanical corneal damage. The central cornea or limbal tissue was excised at different time intervals after injury, and the expression of genes in the explants was determined. It was observed that a number of genes for growth and differentiation factors were significantly upregulated in the cornea rapidly after injury. The ability of these factors to regulate the differentiation and proliferation of limbal cells was tested. It was found that the insulin-like growth factor-I (IGF-I), which is rapidly overexpressed after injury, enhances the expression of IGF receptor in limbal cells and induces the differentiation of LSC into cells expressing the corneal cell marker, cytokeratin K12, without any effect on limbal cell proliferation. In contrast, the epidermal growth factor (EGF) and fibroblast growth factor-beta (FGF-beta), which are also produced by the damaged corneal epithelium, supported limbal cell proliferation without any effect on their differentiation. Other factors did not affect limbal cell differentiation or proliferation. Thus, IGF-I was identified as the main factor stimulating the expression of IGF receptors in limbal cells and inducing the differentiation of LSC into cells expressing corneal epithelial cell markers. The proliferation of these cells was supported by EGF and FGF.
dcterms:title
The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process
skos:prefLabel
The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process The key role of insulin-like growth factor I in limbal stem cell differentiation and the corneal wound-healing process
skos:notation
RIV/68378050:_____/12:00392142!RIV14-GA0-68378050
n18:predkladatel
n19:ico%3A68378050
n3:aktivita
n10:Z n10:S n10:P n10:I
n3:aktivity
I, P(GAP304/11/0653), P(GD310/08/H077), P(KAN200520804), S, Z(MSM0021620858)
n3:cisloPeriodika
18
n3:dodaniDat
n17:2014
n3:domaciTvurceVysledku
n4:5695678 n4:6947891 n4:3650871 n4:2767198 n4:7357354 n4:9565612
n3:druhVysledku
n20:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
144602
n3:idVysledku
RIV/68378050:_____/12:00392142
n3:jazykVysledku
n14:eng
n3:klicovaSlova
IGF-I; limbal stem cells; cornea; healing
n3:klicoveSlovo
n7:cornea n7:healing n7:IGF-I n7:limbal%20stem%20cells
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[AFDFF994245E]
n3:nazevZdroje
Stem Cells and Development
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n9:GD310%2F08%2FH077 n9:GAP304%2F11%2F0653 n9:KAN200520804
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
21
n3:tvurceVysledku
Svobodová, Eliška Holáň, Vladimír Zajícová, Alena Trošan, Peter Chudíčková, Milada Krulová, Magdalena
n3:wos
000311953700010
n3:zamer
n13:MSM0021620858
s:issn
1547-3287
s:numberOfPages
10
n11:doi
10.1089/scd.2012.0180