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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F12%3A00387840%21RIV13-GA0-68378050
rdf:type
skos:Concept n11:Vysledek
dcterms:description
WWe and others have previously shown that increased expression and altered compartmentalization of γ-tubulin may contribute to tumorigenesis and tumor progression (J. Cell Physiol. 2009;223:519-529; Cancer Biol. Ther. 2010;9:66-76). Here we have determined by immunohistochemistry the localization and cellular distribution of γ-tubulin in clinical tissue samples from 109 non-small cell lung cancer (NSCLC) cases. The expression and distribution of γ-tubulin protein and transcripts was also determined in the NSCLC tumor cell lines NCI-H460 (HTB-177) and NCI-H69 (HTB-119) by immunocytochemistry, quantitative immunoblotting and reverse transcription quantitative real-time PCR (RT-qPCR). Polyclonal and monoclonal anti-peptide antibodies recognizing epitopes in the C- or N-terminal domains of γ-tubulins and human gene-specific primers for γ-tubulins 1 (TUBG1) and 2 (TUBG2) were used. In non-neoplastic cells of the airway epithelium in situ, γ-tubulin exhibited predominantly apical surface and pericentriolar localizations. In contrast, markedly increased, albeit heterogeneous and variously prominent γ-tubulin immunoreactivity was detected in clinical tumor specimens and in the NCI-H460 and NCI-H69 cell lines, where tumor cells exhibited overlapping multi-punctate and diffuse patterns of localization. Co-expression of γ-tubulin and Ki-67 (MIB-1) was detected in a population of proliferating tumor cells. A statistically significant increase of γ-tubulin expression was found in Stage III compared to lesser stage tumors (p<0.001 v. Stages I/II) regardless of histological subtype or grade. By quantitative immunoblotting NCI-H460 and NCI-H69 cells expressed higher levels of γ-tubulin protein compared to small airway epithelial cells (SAEC). In both tumor cell lines increase in TUBG1 and TUBG2 transcripts was detected by RT-qPCR. Our results reveal for the first time an increased expression of γ-tubulin in lung cancer. WWe and others have previously shown that increased expression and altered compartmentalization of γ-tubulin may contribute to tumorigenesis and tumor progression (J. Cell Physiol. 2009;223:519-529; Cancer Biol. Ther. 2010;9:66-76). Here we have determined by immunohistochemistry the localization and cellular distribution of γ-tubulin in clinical tissue samples from 109 non-small cell lung cancer (NSCLC) cases. The expression and distribution of γ-tubulin protein and transcripts was also determined in the NSCLC tumor cell lines NCI-H460 (HTB-177) and NCI-H69 (HTB-119) by immunocytochemistry, quantitative immunoblotting and reverse transcription quantitative real-time PCR (RT-qPCR). Polyclonal and monoclonal anti-peptide antibodies recognizing epitopes in the C- or N-terminal domains of γ-tubulins and human gene-specific primers for γ-tubulins 1 (TUBG1) and 2 (TUBG2) were used. In non-neoplastic cells of the airway epithelium in situ, γ-tubulin exhibited predominantly apical surface and pericentriolar localizations. In contrast, markedly increased, albeit heterogeneous and variously prominent γ-tubulin immunoreactivity was detected in clinical tumor specimens and in the NCI-H460 and NCI-H69 cell lines, where tumor cells exhibited overlapping multi-punctate and diffuse patterns of localization. Co-expression of γ-tubulin and Ki-67 (MIB-1) was detected in a population of proliferating tumor cells. A statistically significant increase of γ-tubulin expression was found in Stage III compared to lesser stage tumors (p<0.001 v. Stages I/II) regardless of histological subtype or grade. By quantitative immunoblotting NCI-H460 and NCI-H69 cells expressed higher levels of γ-tubulin protein compared to small airway epithelial cells (SAEC). In both tumor cell lines increase in TUBG1 and TUBG2 transcripts was detected by RT-qPCR. Our results reveal for the first time an increased expression of γ-tubulin in lung cancer.
dcterms:title
Overexpression of γ-tubulin in non-small cell lung cancer Overexpression of γ-tubulin in non-small cell lung cancer
skos:prefLabel
Overexpression of γ-tubulin in non-small cell lung cancer Overexpression of γ-tubulin in non-small cell lung cancer
skos:notation
RIV/68378050:_____/12:00387840!RIV13-GA0-68378050
n11:predkladatel
n12:ico%3A68378050
n3:aktivita
n4:Z n4:P
n3:aktivity
P(GA204/09/1777), P(GAP302/10/1701), P(LC545), Z(AV0Z50520514)
n3:cisloPeriodika
9
n3:dodaniDat
n10:2013
n3:domaciTvurceVysledku
n9:9103813 n9:3093360 n9:7054777
n3:druhVysledku
n17:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
157462
n3:idVysledku
RIV/68378050:_____/12:00387840
n3:jazykVysledku
n13:eng
n3:klicovaSlova
gamma-tubulin; microtubules; NSCLC
n3:klicoveSlovo
n16:microtubules n16:NSCLC n16:gamma-tubulin
n3:kodStatuVydavatele
ES - Španělské království
n3:kontrolniKodProRIV
[83CE3FFAE017]
n3:nazevZdroje
Histology and Histopathology
n3:obor
n6:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
12
n3:projekt
n8:LC545 n8:GA204%2F09%2F1777 n8:GAP302%2F10%2F1701
n3:rokUplatneniVysledku
n10:2012
n3:svazekPeriodika
27
n3:tvurceVysledku
Giordano, A. Riga, D. Caracciolo, V. Dráberová, Eduarda Katsetos, C. D. Trakas, N. Sulimenko, Tetyana Emmanouilidou, A. Mahera, E. Maounis, N. F. Chorti, M. Dráber, Pavel
n3:wos
000306521400007
n3:zamer
n18:AV0Z50520514
s:issn
0213-3911
s:numberOfPages
12