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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F12%3A00375928%21RIV13-GA0-68378050
rdf:type
skos:Concept n9:Vysledek
dcterms:description
While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules. While γ-tubulin 1 is ubiquitous, γ-tubulin 2 is known to be not able to substitute for γ-tubulin 1 deficiency in blastocysts. This research article presents our contribution to deciphering of γ-tubulin 2 role. We have found that exogenous γ-tubulin 2 is targeted to centrosomes and interacts with γ-tubulin complex proteins 2 and 4. Depletion of γ-tubulin 1 by RNAi in U2Os cells causes impaired microtubule nucleation and metaphase arrest. Wild-type phenotype in γ-tubulin 1-depleted cells is restored by expression of exogenous mouse and human γ-tubulin 2. Using RT-qPCR and 2D-PAGE, we have found that Tubg2 expression is dramatically reduced in mouse blastocysts. This indicates that γ-tubulin 2 cannot rescue γ-tubulin 1 deficiency in knock-out blastocysts. The obtained data suggest that γ-tubulin 2 is able to nucleate microtubules and subtitute for γ-tubulin 1. We propose that mammalian γ-tubulins are functionally redundant with respect to the nucleation activity of microtubules.
dcterms:title
γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis
skos:prefLabel
γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis
skos:notation
RIV/68378050:_____/12:00375928!RIV13-GA0-68378050
n9:predkladatel
n14:ico%3A68378050
n3:aktivita
n6:Z n6:P
n3:aktivity
P(1M0506), P(GA204/09/1777), P(GAP302/10/1701), P(GD204/09/H084), P(KAN200520701), P(LC545), Z(AV0Z50520514)
n3:cisloPeriodika
1
n3:dodaniDat
n11:2013
n3:domaciTvurceVysledku
n4:5050383 n4:1205706 n4:2905604 n4:9103813 n4:7054777 n4:9123431 n4:3093360 n4:2983273
n3:druhVysledku
n10:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
183166
n3:idVysledku
RIV/68378050:_____/12:00375928
n3:jazykVysledku
n12:eng
n3:klicovaSlova
γ-tubulin 2; nucleation; embryogenesis
n3:klicoveSlovo
n7:nucleation n7:embryogenesis n7:%CE%B3-tubulin%202
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[DE28A5B4810F]
n3:nazevZdroje
PLoS ONE
n3:obor
n20:EB
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
8
n3:projekt
n15:GA204%2F09%2F1777 n15:GD204%2F09%2FH084 n15:KAN200520701 n15:1M0506 n15:GAP302%2F10%2F1701 n15:LC545
n3:rokUplatneniVysledku
n11:2012
n3:svazekPeriodika
7
n3:tvurceVysledku
Sulimenko, Vadym Dráberová, Eduarda Sulimenko, Tetyana Vosecká, Věra Vinopal, Stanislav Černohorská, Markéta Flemr, Matyáš Dráber, Pavel
n3:wos
000301123400112
n3:zamer
n18:AV0Z50520514
s:issn
1932-6203
s:numberOfPages
14
n5:doi
10.1371/journal.pone.0029919