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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F11%3A00371215%21RIV12-AV0-68378050
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Leishmanias are causative agents of leishmaniases and an important biological model for study host-pathogen interaction. Many methods of their cultivation on biphasic and liquid media are available. Biphasic media are superior for initial isolation of parasites and obtaining high promastigote infectivity; liquid media are more suitable for large-scale experiments. Our study adapted and optimized cultivation of Leishmania promastigotes on biphasic SNB-9 (saline–neopeptone–blood 9) originally developed for Trypanosoma cultivation, combining advantages of biphasic and liquid media. We demonstrate that SNB-9 considerably surpasses Schneider's Insect Medium in in vitro infectivity of the parasites and show that the ratio of apoptotic parasites important for the infectivity of the inoculum is higher in SNB-9 culture than in Schneider's Insect Medium culture. Cultivation of Leishmania on SNB-9 thus reliably yields highly infective promastigotes suitable for experimental infection. Leishmanias are causative agents of leishmaniases and an important biological model for study host-pathogen interaction. Many methods of their cultivation on biphasic and liquid media are available. Biphasic media are superior for initial isolation of parasites and obtaining high promastigote infectivity; liquid media are more suitable for large-scale experiments. Our study adapted and optimized cultivation of Leishmania promastigotes on biphasic SNB-9 (saline–neopeptone–blood 9) originally developed for Trypanosoma cultivation, combining advantages of biphasic and liquid media. We demonstrate that SNB-9 considerably surpasses Schneider's Insect Medium in in vitro infectivity of the parasites and show that the ratio of apoptotic parasites important for the infectivity of the inoculum is higher in SNB-9 culture than in Schneider's Insect Medium culture. Cultivation of Leishmania on SNB-9 thus reliably yields highly infective promastigotes suitable for experimental infection.
dcterms:title
Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium
skos:prefLabel
Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium Preparation of highly infective Leishmania promastigotes by cultivation on SNB-9 biphasic medium
skos:notation
RIV/68378050:_____/11:00371215!RIV12-AV0-68378050
n10:predkladatel
n17:ico%3A68378050
n3:aktivita
n8:S n8:P n8:Z
n3:aktivity
P(GA310/08/1697), P(LC06009), S, Z(AV0Z50520514)
n3:cisloPeriodika
3
n3:dodaniDat
n16:2012
n3:domaciTvurceVysledku
n11:6111122 n11:5279097
n3:druhVysledku
n12:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
223079
n3:idVysledku
RIV/68378050:_____/11:00371215
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Leishmania; SNB-9; biphasic culture medium; parasite infectivity
n3:klicoveSlovo
n4:biphasic%20culture%20medium n4:parasite%20infectivity n4:Leishmania n4:SNB-9
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[AE88BA1E8726]
n3:nazevZdroje
Journal of Microbiological Methods
n3:obor
n19:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n14:LC06009 n14:GA310%2F08%2F1697
n3:rokUplatneniVysledku
n16:2011
n3:svazekPeriodika
87
n3:tvurceVysledku
Svobodová, M. Grekov, Igor Lipoldová, Marie Nohýnková, E.
n3:wos
000297892200003
n3:zamer
n18:AV0Z50520514
s:issn
0167-7012
s:numberOfPages
5
n15:doi
10.1016/j.mimet.2011.08.012