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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F11%3A00355821%21RIV11-MSM-68378050
rdf:type
n13:Vysledek skos:Concept
dcterms:description
The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry. The best available approach of biological sample preparation for transmission electron microscopy currently includes cryoimmobilization by high-pressure freezing (HPF) followed by freeze-substitution (FS). This method has been well established for interphase cells; however, a reliable and easy procedure is still missing for mitotic cells especially because of their fragility and sensitivity to treatments. Here, we present a fast and effective method for HPF/automated FS and LR White embedding of mitotic cells which allows for their controlled and reproducible quality processing. It should be useful in various ultrastructural studies on mitotic cells especially in combination with immunocytochemistry.
dcterms:title
A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin
skos:prefLabel
A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin A method for preserving ultrastructural properties of mitotic cells for subsequent immunogold labeling using low-temperature embedding in LR White resin
skos:notation
RIV/68378050:_____/11:00355821!RIV11-MSM-68378050
n13:predkladatel
n19:ico%3A68378050
n3:aktivita
n4:Z n4:P
n3:aktivity
P(2B06063), P(KAN200520704), P(LC545), Z(AV0Z50520514), Z(AV0Z60220518)
n3:cisloPeriodika
1
n3:dodaniDat
n16:2011
n3:domaciTvurceVysledku
n5:6055419 n5:9671706
n3:druhVysledku
n7:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
183883
n3:idVysledku
RIV/68378050:_____/11:00355821
n3:jazykVysledku
n8:eng
n3:klicovaSlova
Mitotic cells; High-pressure freezing; Histochemistry
n3:klicoveSlovo
n11:Histochemistry n11:High-pressure%20freezing n11:Mitotic%20cells
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[B54A1C77F70B]
n3:nazevZdroje
Histochemistry and Cell Biology
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n10:KAN200520704 n10:2B06063 n10:LC545
n3:rokUplatneniVysledku
n16:2011
n3:svazekPeriodika
135
n3:tvurceVysledku
Sobol, Margaryta Hozák, Pavel Nebesářová, Jana
n3:wos
000286794000011
n3:zamer
n14:AV0Z50520514 n14:AV0Z60220518
s:issn
0948-6143
s:numberOfPages
8