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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F10%3A00351320%21RIV11-GA0-68378050
rdf:type
n10:Vysledek skos:Concept
dcterms:description
Nuclear actin and nuclear myosin I (NMI) are important players in transcription of ribosomal genes. Transcription of rDNA takes place in highly organized intranuclear compartment, the nucleolus. In this study, we characterized the localization of these two proteins within the nucleolus of HeLa cells with high structural resolution by means of electron microscopy and gold-immunolabeling. We demonstrate that both actin and NMI are localized in specific compartments within the nucleolus, and the distribution of NMI is transcription-dependent. Moreover, a pool of NMI is present in the foci containing nascent rRNA transcripts. Actin, in turn, is present both in transcriptionally active and inactive regions of the nucleolus and colocalizes with RNA polymerase I and UBF. Our data support the involvement of actin and NMI in rDNA transcription and point out to other functions of these proteins in the nucleolus, such as rRNA maturation and maintenance of nucleolar architecture. Nuclear actin and nuclear myosin I (NMI) are important players in transcription of ribosomal genes. Transcription of rDNA takes place in highly organized intranuclear compartment, the nucleolus. In this study, we characterized the localization of these two proteins within the nucleolus of HeLa cells with high structural resolution by means of electron microscopy and gold-immunolabeling. We demonstrate that both actin and NMI are localized in specific compartments within the nucleolus, and the distribution of NMI is transcription-dependent. Moreover, a pool of NMI is present in the foci containing nascent rRNA transcripts. Actin, in turn, is present both in transcriptionally active and inactive regions of the nucleolus and colocalizes with RNA polymerase I and UBF. Our data support the involvement of actin and NMI in rDNA transcription and point out to other functions of these proteins in the nucleolus, such as rRNA maturation and maintenance of nucleolar architecture.
dcterms:title
Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
skos:prefLabel
Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
skos:notation
RIV/68378050:_____/10:00351320!RIV11-GA0-68378050
n3:aktivita
n5:P n5:Z
n3:aktivity
P(2B06063), P(GA204/07/1592), P(LC545), P(ME09101), Z(AV0Z50110509), Z(AV0Z50520514)
n3:cisloPeriodika
3
n3:dodaniDat
n8:2011
n3:domaciTvurceVysledku
n13:9671706 n13:2939711
n3:druhVysledku
n9:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
293277
n3:idVysledku
RIV/68378050:_____/10:00351320
n3:jazykVysledku
n18:eng
n3:klicovaSlova
RNA-polymerase-I; ultrastructural analysis; UBF
n3:klicoveSlovo
n16:ultrastructural%20analysis n16:UBF n16:RNA-polymerase-I
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[10DB9E6271C1]
n3:nazevZdroje
Histochemistry and Cell Biology
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n15:ME09101 n15:GA204%2F07%2F1592 n15:LC545 n15:2B06063
n3:rokUplatneniVysledku
n8:2010
n3:svazekPeriodika
134
n3:tvurceVysledku
Janáček, Jiří Philimonenko, Vlada Harata, M. Hozák, Pavel
n3:wos
000281249500002
n3:zamer
n14:AV0Z50110509 n14:AV0Z50520514
s:issn
0948-6143
s:numberOfPages
7