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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F09%3A00336266%21RIV10-MSM-68378050
rdf:type
skos:Concept n21:Vysledek
dcterms:description
Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes. Various resins (Lowicryl, LR White, LR Gold, Unicryl) used for embedding were compared for their ability to reveal antigens for immunogold labelling on their surface. Their surfaces after cutting with a diamond knife were observed using atomic force microscopy, and results statistically analyzed. The LR White resin in combination with HPF/FS was shown to be able to be successfully used for fine ultrastructural immunocytochemistry. Methods were presented allowing detection of clustering or colocalization of antigens/gold particles. A complete set of tools is now available for reliable detection of gold particles in EM images, their statistical evaluation and production of convenient outputs for publication of results. The plug-ins are useful addition to image analysis software accompanying modern CCD cameras for electron microscopes.
dcterms:title
Optimalization of immunogold labeling on thin resin sections after cryofixation Optimalization of immunogold labeling on thin resin sections after cryofixation
skos:prefLabel
Optimalization of immunogold labeling on thin resin sections after cryofixation Optimalization of immunogold labeling on thin resin sections after cryofixation
skos:notation
RIV/68378050:_____/09:00336266!RIV10-MSM-68378050
n4:aktivita
n6:Z n6:P
n4:aktivity
P(2B06063), P(GD204/09/H084), P(KAN200520704), P(LC545), Z(AV0Z40400503), Z(AV0Z50110509), Z(AV0Z50520514)
n4:dodaniDat
n8:2010
n4:domaciTvurceVysledku
n7:9671706 n7:6055419 n7:6029876 n7:2939711
n4:druhVysledku
n12:D
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
331969
n4:idVysledku
RIV/68378050:_____/09:00336266
n4:jazykVysledku
n15:eng
n4:klicovaSlova
cell ultrastructure; high pressure freezing; freeze substitutions; resins; immunocytochemistry
n4:klicoveSlovo
n5:resins n5:freeze%20substitutions n5:high%20pressure%20freezing n5:immunocytochemistry n5:cell%20ultrastructure
n4:kontrolniKodProRIV
[FCF0508FCCAE]
n4:mistoKonaniAkce
Graz
n4:mistoVydani
Graz
n4:nazevZdroje
MC 2009 - Microscopy Conference: First Joint Meeting of Dreiländertagung and Multinational Conference on Microscopy
n4:obor
n11:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
8
n4:projekt
n9:2B06063 n9:KAN200520704 n9:GD204%2F09%2FH084 n9:LC545
n4:rokUplatneniVysledku
n8:2009
n4:tvurceVysledku
Philimonenko, Anatolij Janda, Pavel Janáček, Jiří Hozák, Pavel Vyhnal, Aleš Sobol, Margaryta Philimonenko, Vlada Kubínová, Lucie
n4:typAkce
n14:WRD
n4:zahajeniAkce
2009-08-30+02:00
n4:zamer
n10:AV0Z50520514 n10:AV0Z40400503 n10:AV0Z50110509
s:numberOfPages
2
n20:hasPublisher
Verlag der Technischen Universität
n17:isbn
978-3-85125-062-6