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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F09%3A00334131%21RIV10-AV0-68378050
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells. Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells.
dcterms:title
Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy
skos:prefLabel
Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy
skos:notation
RIV/68378050:_____/09:00334131!RIV10-AV0-68378050
n3:aktivita
n5:Z n5:P
n3:aktivity
P(GA202/08/0590), Z(AV0Z50520514)
n3:cisloPeriodika
6
n3:dodaniDat
n6:2010
n3:domaciTvurceVysledku
n17:2631334
n3:druhVysledku
n11:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
337412
n3:idVysledku
RIV/68378050:_____/09:00334131
n3:jazykVysledku
n14:eng
n3:klicovaSlova
tumour cell; nutritional deprivation; energy deprivation; cell survival; cell death; digital holographic microscopy; dynamic phase difference
n3:klicoveSlovo
n9:digital%20holographic%20microscopy n9:dynamic%20phase%20difference n9:cell%20death n9:tumour%20cell n9:cell%20survival n9:nutritional%20deprivation n9:energy%20deprivation
n3:kodStatuVydavatele
GR - Řecká republika
n3:kontrolniKodProRIV
[F9FF58792F56]
n3:nazevZdroje
Anticancer Research
n3:obor
n12:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n16:GA202%2F08%2F0590
n3:rokUplatneniVysledku
n6:2009
n3:svazekPeriodika
29
n3:tvurceVysledku
Janečková, H. Chmelík, R. Veselý, Pavel
n3:wos
000267389500061
n3:zamer
n8:AV0Z50520514
s:issn
0250-7005
s:numberOfPages
7