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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F09%3A00334099%21RIV10-MSM-68378050
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA–protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA–protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA–protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA–protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA–protein complexes. Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA–protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA–protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA–protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using two different approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA–protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA–protein complexes.
dcterms:title
In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer
skos:prefLabel
In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer
skos:notation
RIV/68378050:_____/09:00334099!RIV10-MSM-68378050
n3:aktivita
n6:P n6:Z
n3:aktivity
P(KAN200520801), P(LC06063), Z(AV0Z40400503), Z(AV0Z50520514)
n3:cisloPeriodika
11
n3:dodaniDat
n8:2010
n3:domaciTvurceVysledku
n4:3556654 n4:8466572
n3:druhVysledku
n11:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
318932
n3:idVysledku
RIV/68378050:_____/09:00334099
n3:jazykVysledku
n18:eng
n3:klicovaSlova
FRET; FLIM; RNA-protein interactions
n3:klicoveSlovo
n15:RNA-protein%20interactions n15:FLIM n15:FRET
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[DC128D3C8B2E]
n3:nazevZdroje
R N A
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n9:KAN200520801 n9:LC06063
n3:rokUplatneniVysledku
n8:2009
n3:svazekPeriodika
15
n3:tvurceVysledku
Jablonski, J. A Huranová, Martina Benda, Aleš Staněk, David Hof, Martin Caputi, M.
n3:wos
000270851000014
n3:zamer
n13:AV0Z50520514 n13:AV0Z40400503
s:issn
1355-8382
s:numberOfPages
9