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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F05%3A00027620%21RIV06-AV0-68378050
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Do mezinárodního workshopu HLDA8 bylo zasláno přes 600 protilátek. Z nich 101 bylo vybráno do “slepého panelu”, který obsahoval také 5 negativních kontrol a 27 pozitivních kontrol známých CD specificit. Pro 31 vzorků byly identifikovány jejich specifity (známé CD a MHC proteiny). Bylo identifikováno několik párů velmi podobných vzorků, které pak byly srovnávány co do identity cílových antigenů pomocí Western blottingu a imunohistochemie. There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity.
dcterms:title
The HLDA8 blind panel: findings and conclusions The HLDA8 blind panel: findings and conclusions Slepý panel HLDA8: poznatky a závěry
skos:prefLabel
The HLDA8 blind panel: findings and conclusions Slepý panel HLDA8: poznatky a závěry The HLDA8 blind panel: findings and conclusions
skos:notation
RIV/68378050:_____/05:00027620!RIV06-AV0-68378050
n3:strany
75;83
n3:aktivita
n8:Z
n3:aktivity
Z(AV0Z5052915)
n3:cisloPeriodika
1
n3:dodaniDat
n5:2006
n3:domaciTvurceVysledku
n12:9399062 n12:7776535 n12:9395970
n3:druhVysledku
n15:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
523441
n3:idVysledku
RIV/68378050:_____/05:00027620
n3:jazykVysledku
n14:eng
n3:klicovaSlova
CD molecules; leukocyte antigen
n3:klicoveSlovo
n4:CD%20molecules n4:leukocyte%20antigen
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[28E03DCCBB91]
n3:nazevZdroje
Journal of Immunological Methods
n3:obor
n10:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
17
n3:rokUplatneniVysledku
n5:2005
n3:svazekPeriodika
305
n3:tvurceVysledku
Zola, H. Drbal, Karel Millard, D. Macardle, P. Angelisová, Pavla Milford, E. L. Tinckam, K. Salganik, M. P. Wand, M. P. Hollemweguer, E. Bailey, S. Swart, B. Nairn, J. Hořejší, Václav Dagdeviren, A. Dandie, G. W. Hodge, G.
n3:zamer
n13:AV0Z5052915
s:issn
0022-1759
s:numberOfPages
9