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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F05%3A00000229%21RIV%2F2005%2FAV0%2FA23005%2FN
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs. Gen Hybrid sterility 1 (Hst1) ovlivňuje fertilitu hybridních samců mezi některými laboratorními kmeny (např. C57BL/10) a myší (pod)druhu Mus musculus musculus. Během pozičního klonování Hst1 genu jsme vygenerovali kontig bakteriálních artificiálních chromosomů (BAC) a vzorkovou sekvenci kandidátní oblasti ze 129S1/SvImJ kmene. Nové genetické markery nám umožnily zúžit Hst1 oblast z 580 na 360 kb. Produkty dvou genů z této oblasti, TATA-vazebný protein (Tbp) a proteasomová podjednotka beta 1 (Psmb1), akumulují během spermatogeneze. Tyto proteiny mají konzervované C-terminalní sekvence a druhově-specifické N-konce. Vyhodnotili jsme kandidaturu těchto genů na gen Hst1 alelickým sekvenováním a semikvantitativním RT PCR v reálném čase pro jejich testikulární mRNA. The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs.
dcterms:title
Poziční klonování genu Hybrid sterility 1: genetické mapování a evaluace dvou kandidátních genů Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes
skos:prefLabel
Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes Poziční klonování genu Hybrid sterility 1: genetické mapování a evaluace dvou kandidátních genů
skos:notation
RIV/68378050:_____/05:00000229!RIV/2005/AV0/A23005/N
n3:strany
637;641
n3:aktivita
n9:P n9:Z
n3:aktivity
P(GA204/01/0997), P(GV204/98/K015), P(LN00A079), Z(AV0Z50520514), Z(AV0Z5052915)
n3:cisloPeriodika
-
n3:dodaniDat
n4:2005
n3:domaciTvurceVysledku
n6:4143086 n6:6662102 n6:3144577 n6:7615388 n6:2894297
n3:druhVysledku
n10:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
536898
n3:idVysledku
RIV/68378050:_____/05:00000229
n3:jazykVysledku
n13:eng
n3:klicovaSlova
house mouse; Mus musculus; speciation
n3:klicoveSlovo
n14:house%20mouse n14:speciation n14:Mus%20musculus
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[9138793B8974]
n3:nazevZdroje
Biological Journal of the Linnean Society
n3:obor
n15:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n12:GA204%2F01%2F0997 n12:LN00A079 n12:GV204%2F98%2FK015
n3:rokUplatneniVysledku
n4:2005
n3:svazekPeriodika
84
n3:tvurceVysledku
Vlček, Čestmír Himmelbauer, H. Mihola, Ondřej Trachtulec, Zdeněk Pačes, Václav Forejt, Jiří
n3:zamer
n17:AV0Z50520514 n17:AV0Z5052915
s:issn
0024-4066
s:numberOfPages
5