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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F04%3A00105370%21RIV%2F2005%2FGA0%2FA23005%2FN
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Studium specifických a funkčně aktivních antigenů spermií by mohlo napomoci objasnit mechanismy interakce gamet a najít nové postupu prognózy a regulace plodnosti. Využili jsme polyklonální králičí protikančí protilátku (RABSA) k dvojité imunodifúzi, elektroforéze, imunofluorescenčnímu testu a testu ELISA. Protilátka RABSA barvila akrozomy. RABSA je specificky lokalizován ve spermatických strukturách. U Westernova přenosu rozpoznávala RABSA specificky antigen s molekulovou hmotností 60 kDa (Ag60). Biologické a IIF experimenty prokazují, že Ag60 se účastní funkčních kroků nezbytných pro vazbu spermie na vajíčko a/nebo jejich fúzi, ale ne pro vazbu spermie na zonu pellucidu Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding Investigations on specific and functionally active sperm antigens would bring about the elucidation of the mechanisms of gamete interaction and help the search to new approaches for prognosis and regulation of fertility.We used polyclonal rabbit anti-boar spermatozoa antibody (RABSA)by double immunodiffusion, electrophoresis, immunofluorescence test and ELISA. The RABSA stained acrosomes. The RABSA is specifically localized in sperm structures. In WB RABSA recognized specifically antigen with molecular mass 60 kDa (Ag60). The biological and IIF experiments provide evidence supporting the involvement of Ag60 in functional steps required for sperm-egg binding and/or fusion, but not sperm-zona pellucida binding
dcterms:title
Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identifikace a izolace kančích antigenů specifických pro spermie s potenciální úlohou v interakci spermie s vajíčkem
skos:prefLabel
Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identification and isolation of boar sperm specific antigens with potential role in sperm-egg interaction Identifikace a izolace kančích antigenů specifických pro spermie s potenciální úlohou v interakci spermie s vajíčkem
skos:notation
RIV/68378050:_____/04:00105370!RIV/2005/GA0/A23005/N
n3:strany
91;106
n3:aktivita
n9:Z n9:P
n3:aktivity
P(GA204/02/1373), P(NJ7463), Z(AV0Z5052915)
n3:cisloPeriodika
1-2
n3:dodaniDat
n15:2005
n3:domaciTvurceVysledku
n6:4192567
n3:druhVysledku
n17:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
567134
n3:idVysledku
RIV/68378050:_____/04:00105370
n3:jazykVysledku
n13:eng
n3:klicovaSlova
sperm-egg interaction; antibodies
n3:klicoveSlovo
n4:antibodies n4:sperm-egg%20interaction
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[0BD1DDFD6626]
n3:nazevZdroje
Journal of Reproductive Immunology
n3:obor
n16:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n7:GA204%2F02%2F1373 n7:NJ7463
n3:rokUplatneniVysledku
n15:2004
n3:svazekPeriodika
64
n3:tvurceVysledku
Stamenova, M. Baracova, V. Mollova, M. Ivanova, M. Pěknicová, Jana
n3:zamer
n18:AV0Z5052915
s:issn
0165-0378
s:numberOfPages
16