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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F03%3A23033225%21RIV%2F2004%2FGA0%2FA23004%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron. In the fifth intron of the Mus musculus oncogene src a complex microsatellite was discovered that consists of a CA run and 31 bp apart followed by track of TG dinucleotides interrupted by extra Gs in specific intervals. As the 31 bp interlink can assume a hairpin structure, the whole region can be viewed as an imperfect inverted repeat. The microsatellite was sequenced in imbred lines of M. m. musculus and M. m. domesticus, as well as in mice of both subspecies trapped near the hybrid zone. Differences in the length of repeat regions, insertion/deletions and simple base changes characterize the strains. Hybrids between the two species could be identified by sequencing of cloned PCR products. The structure of the microsatellite in more distant species was found to be conspicuously different. Instead of frequent (CA)18-25, we can see only (CA)8 in M. spretus, CATGCGCCCCCCA in Mus pahari and CACCCCCC in Mus caroli. No similar structure is present in the corresponding human src intron.
dcterms:title
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution. Microsatellite region in the murine oncogene src - its primary structure and variability during evolution.
skos:prefLabel
Microsatellite region in the murine oncogene src - its primary structure and variability during evolution. Microsatellite region in the murine oncogene src - its primary structure and variability during evolution.
skos:notation
RIV/68378050:_____/03:23033225!RIV/2004/GA0/A23004/N
n3:strany
93
n3:aktivita
n17:Z n17:P
n3:aktivity
P(GA301/02/0408), Z(AV0Z5052915)
n3:dodaniDat
n10:2004
n3:domaciTvurceVysledku
n4:4603478 n4:6962726 n4:9802827 n4:3886875 n4:8802955
n3:druhVysledku
n8:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
615419
n3:idVysledku
RIV/68378050:_____/03:23033225
n3:jazykVysledku
n6:eng
n3:klicovaSlova
Mus musculus, c-src introns, microsatellite
n3:klicoveSlovo
n7:Mus%20musculus n7:microsatellite n7:c-src%20introns
n3:kontrolniKodProRIV
[8DB252357079]
n3:mistoKonaniAkce
Praha [CZ]
n3:mistoVydani
Praha
n3:nazevZdroje
Functional Genomics a Disease, Final programme and Abstract book.
n3:obor
n14:EB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n13:GA301%2F02%2F0408
n3:rokUplatneniVysledku
n10:2003
n3:tvurceVysledku
Fučík, Vladimír Ferjentsik, Zoltán Jonák, Jiří Beran, Jaroslav Šindelka, Radek
n3:typAkce
n18:WRD
n3:zahajeniAkce
2003-05-14+02:00
n3:zamer
n11:AV0Z5052915
s:numberOfPages
1
n20:hasPublisher
ESF IN FUNCTIONAL GENOMICS