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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F03%3A23033178%21RIV%2F2004%2FAV0%2FA23004%2FN
rdf:type
skos:Concept n14:Vysledek
dcterms:description
The transcriptional activity of an integrated retroviral copy strongly depends on the adjacent host-cell DNA at the site of integration. Transcribed DNA loci as well as cis-acting sequences like enhancers or CpG islands usually permit expression of nearby integrated proviruses. In contrast, proviruses residing close to cellular silencers tend to transcriptional silencing and CpG methylation. Little is known, however, about the influence of provirus integration on the target sequence in the host genome. Here, we report interesting features of a simplified Rous sarcoma virus integrated into a non-transcribed hypermethylated DNA sequence in the Syrian hamster genome. After integration, CpG methylation of this sequence has been lost almost completely and hypomethylated DNA permits proviral transcription and hamster cell transformation by the proviral v-src oncogene. This, however, is not a stable state, and non-transformed revertants bearing transcriptionally silenced proviruses segregate with a high rate The transcriptional activity of an integrated retroviral copy strongly depends on the adjacent host-cell DNA at the site of integration. Transcribed DNA loci as well as cis-acting sequences like enhancers or CpG islands usually permit expression of nearby integrated proviruses. In contrast, proviruses residing close to cellular silencers tend to transcriptional silencing and CpG methylation. Little is known, however, about the influence of provirus integration on the target sequence in the host genome. Here, we report interesting features of a simplified Rous sarcoma virus integrated into a non-transcribed hypermethylated DNA sequence in the Syrian hamster genome. After integration, CpG methylation of this sequence has been lost almost completely and hypomethylated DNA permits proviral transcription and hamster cell transformation by the proviral v-src oncogene. This, however, is not a stable state, and non-transformed revertants bearing transcriptionally silenced proviruses segregate with a high rate
dcterms:title
Demethylation of host-cell DNA at the site of avian retrovirus integration. Demethylation of host-cell DNA at the site of avian retrovirus integration.
skos:prefLabel
Demethylation of host-cell DNA at the site of avian retrovirus integration. Demethylation of host-cell DNA at the site of avian retrovirus integration.
skos:notation
RIV/68378050:_____/03:23033178!RIV/2004/AV0/A23004/N
n3:strany
641;648
n3:aktivita
n6:Z
n3:aktivity
Z(AV0Z5052915)
n3:cisloPeriodika
311
n3:dodaniDat
n8:2004
n3:domaciTvurceVysledku
n4:4364090 n4:1262432 n4:3720284 n4:5103215
n3:druhVysledku
n17:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
603029
n3:idVysledku
RIV/68378050:_____/03:23033178
n3:jazykVysledku
n16:eng
n3:klicovaSlova
DNA methylation and demethylation; integration of retroviruses; gene silencing
n3:klicoveSlovo
n7:integration%20of%20retroviruses n7:gene%20silencing n7:DNA%20methylation%20and%20demethylation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[39E72E14F13B]
n3:nazevZdroje
Biochemical and biophysical research vommunications
n3:obor
n9:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:rokUplatneniVysledku
n8:2003
n3:svazekPeriodika
2003
n3:tvurceVysledku
Blažková, Jana Walter, J. Hájková, P. Hejnar, Jiří Elleder, Daniel Svoboda, Jan
n3:zamer
n15:AV0Z5052915
s:issn
0006-291X
s:numberOfPages
8