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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F03%3A23033070%21RIV%2F2004%2FAV0%2FA23004%2FN
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Treatment of cells with the cAMP-elevating agent prostaglandin E2 (PGE2) augmented the level of Cbp/PAG phosphorylation with a concomitant increase in amounts of Csk bound to Cbp/PAG. While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE2 reduced Csk dissociation upon TCR triggering. This correlated with lowered TCR-induced phosphorylation of CD3 zeta-chain and linker for activation of T cells. Moreover, competition of endogenous Csk from lipid rafts abolished PGE2-mediated inhibition of TCR-induced zeta-chain phosphorylation and activation of NFAT and AP-1. Finally, raft-associated Csk already activated via Cbp/PAG binding, gained additional increase in phosphotransferase activity upon protein kinase A-mediated phosphorylation of Csk. It is proposed that cAMP regulates Csk via both spatial and enzymatic mechanisms, thereby inhibiting signaling through the TCR. Treatment of cells with the cAMP-elevating agent prostaglandin E2 (PGE2) augmented the level of Cbp/PAG phosphorylation with a concomitant increase in amounts of Csk bound to Cbp/PAG. While TCR-triggering resulted in transient dissociation of Csk from Cbp/PAG/rafts allowing TCR-induced tyrosine phosphorylation to occur, pretreatment with PGE2 reduced Csk dissociation upon TCR triggering. This correlated with lowered TCR-induced phosphorylation of CD3 zeta-chain and linker for activation of T cells. Moreover, competition of endogenous Csk from lipid rafts abolished PGE2-mediated inhibition of TCR-induced zeta-chain phosphorylation and activation of NFAT and AP-1. Finally, raft-associated Csk already activated via Cbp/PAG binding, gained additional increase in phosphotransferase activity upon protein kinase A-mediated phosphorylation of Csk. It is proposed that cAMP regulates Csk via both spatial and enzymatic mechanisms, thereby inhibiting signaling through the TCR.
dcterms:title
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling.
skos:prefLabel
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling.
skos:notation
RIV/68378050:_____/03:23033070!RIV/2004/AV0/A23004/N
n3:strany
17597;17600
n3:aktivita
n5:P n5:Z
n3:aktivity
P(LN00A026), Z(AV0Z5052915)
n3:cisloPeriodika
20
n3:dodaniDat
n14:2004
n3:domaciTvurceVysledku
n6:7776535
n3:druhVysledku
n11:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
601575
n3:idVysledku
RIV/68378050:_____/03:23033070
n3:jazykVysledku
n13:eng
n3:klicovaSlova
cAMP; Csk; T cell activation
n3:klicoveSlovo
n12:Csk n12:cAMP n12:T%20cell%20activation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[844BF2A1950E]
n3:nazevZdroje
Journal of Biological Chemistry
n3:obor
n16:EC
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n8:LN00A026
n3:rokUplatneniVysledku
n14:2003
n3:svazekPeriodika
278
n3:tvurceVysledku
Tasken, K. Myklebust, S. Hořejší, Václav Abrahamsen, H. Vang, T.
n3:zamer
n17:AV0Z5052915
s:issn
0021-9258
s:numberOfPages
4