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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F02%3A23033121%21RIV%2F2004%2FGA0%2FA23004%2FN
rdf:type
n16:Vysledek skos:Concept
dcterms:description
Proteins of boar prostet secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding and heparin-binding protein fractions. These fractions were thwn subjected to RP HPLC. Elution profiles of fractions of prostate secretion were compared with non-heparin-binding and heparin-binding protein fractions of seminal plasma. Besides, the isolated proteins were characterized by SDS-PAGE. In protein fractions of prostate secretion, PSP I, PSP II, AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the countrary, beta-microseminoprotein was identified in abundant amounts in prostate secretion. Some seminal plasma proteins originating mainly inseminal vesicles could also be secreted by the prostatic gland. Beta-microseminoprotein was found to be produced mainly by the prostate. Proteins of boar prostet secretion were separated by affinity chromatography on heparin-polyacrylamide to non-heparin-binding and heparin-binding protein fractions. These fractions were thwn subjected to RP HPLC. Elution profiles of fractions of prostate secretion were compared with non-heparin-binding and heparin-binding protein fractions of seminal plasma. Besides, the isolated proteins were characterized by SDS-PAGE. In protein fractions of prostate secretion, PSP I, PSP II, AQN 2 and AWN 1 spermadhesins were found in substantially lower amounts than in seminal plasma. On the countrary, beta-microseminoprotein was identified in abundant amounts in prostate secretion. Some seminal plasma proteins originating mainly inseminal vesicles could also be secreted by the prostatic gland. Beta-microseminoprotein was found to be produced mainly by the prostate.
dcterms:title
Isolation of non-heprin-binding and heparin-binding proteins of boar prostate. Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.
skos:prefLabel
Isolation of non-heprin-binding and heparin-binding proteins of boar prostate. Isolation of non-heprin-binding and heparin-binding proteins of boar prostate.
skos:notation
RIV/68378050:_____/02:23033121!RIV/2004/GA0/A23004/N
n3:strany
137;143
n3:aktivita
n12:P n12:Z
n3:aktivity
P(GA303/99/0357), P(GV524/96/K162), Z(AV0Z5052915)
n3:cisloPeriodika
N/A
n3:dodaniDat
n4:2004
n3:domaciTvurceVysledku
n10:7190573 n10:2430304
n3:druhVysledku
n13:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
649801
n3:idVysledku
RIV/68378050:_____/02:23033121
n3:jazykVysledku
n11:eng
n3:klicovaSlova
isolation; prostatic proteins; heparin
n3:klicoveSlovo
n9:heparin n9:isolation n9:prostatic%20proteins
n3:kodStatuVydavatele
IE - Irsko
n3:kontrolniKodProRIV
[4355BB97C68D]
n3:nazevZdroje
Journal of Chromatography B
n3:obor
n8:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n15:GV524%2F96%2FK162 n15:GA303%2F99%2F0357
n3:rokUplatneniVysledku
n4:2002
n3:svazekPeriodika
770
n3:tvurceVysledku
Maňásková, Pavla Liberda, J. Jonáková, Věra Tichá, M.
n3:zamer
n18:AV0Z5052915
s:issn
1570-0232
s:numberOfPages
7