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Statements

Subject Item
n2:RIV%2F68378050%3A_____%2F01%3A00105389%21RIV%2F2005%2FAV0%2FA23005%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5Ž-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5Ž-untranslated part of the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro... Whey acidic protein, WAP, is being recognized as a principal milk protein expressed in pregnant or lactating females of the several mammalian species. Recently it has been shown that 6.3kb 5Ž-untranslated region of the rabbit WAP (rWAP) gene is able to control, and almost completely restrict, the expression of transgene into the mammary gland of the transgenic animal. We cloned genomic fragment carrying the rWAP gene locus from the ?DASHII phage genomic library and used 8.5 kb long 5Ž-untranslated part of the rWAP gene to target the expression of the human erythropoietin gene (hEPO), cloned from the ?FIXII phage genomic library, into the mammary gland of the mouse. Vectors, carrying the hEPO gene or the rWAP-hEPO hybrid gene construct, were injected into the mice ova and 12 transgenic animals were identified by PCR and Southern blot from the progeny of 168 tested littermates. Transgenic mice were viable, fertile and have a normal development. Recombinant human erythropoietin was pro... Kyselý protein syrovátky, WAP, se považuje za nejdůležitější mléčný protein exprimovaný v březích či kojících samic u řady savčích druhů. Nedávno bylo prokázáno, že 5´-netranslatovaná oblast králičího genu pro WAP (rWAP) o 6,3 kb může řídit téměř zcela omezit expresi transgenu v mléčné žláze trangenního zvířete. Klonovali jsme fragment genomu obsahující lokus genu rWAP z fágové genomové knihovny ?DASHII a využili jsme 8,5 kb dlouhou 5´-netranslatovanou část genu rWAP k cílení exprese lidského genu pro erytropoietin (hEPO), klonovaného z fágové genomové knihovny ?FIXII, do mléčné žlázy myši. Vektory obsahující gen pro hEPO či hybridní genový konstrukt rWAP-hEPO byly injikovány do myších vajíček a pomocí PCR a Southernova přenosu bylo mezi 168 testovanými potomky identifikováno 12 transgenních zvířat. Transgenní myší byly životaschopné, plodné a normálně se vyvíjely. Rekombinantní lidský erytropoietin byl produkován v mléce transgenní myší samice na úrovni sekrece 5,345 mIU/ml, jak by...
dcterms:title
Expression of human erythropoietin gene in the mammary gland of a transgenic mouse Expression of human erythropoietin gene in the mammary gland of a transgenic mouse Exprese genu pro lidský erytropoietin v mléčné žláze transgenní myši
skos:prefLabel
Exprese genu pro lidský erytropoietin v mléčné žláze transgenní myši Expression of human erythropoietin gene in the mammary gland of a transgenic mouse Expression of human erythropoietin gene in the mammary gland of a transgenic mouse
skos:notation
RIV/68378050:_____/01:00105389!RIV/2005/AV0/A23005/N
n4:strany
187;195
n4:aktivita
n12:Z
n4:aktivity
Z(AV0Z5052915)
n4:cisloPeriodika
6
n4:dodaniDat
n17:2005
n4:domaciTvurceVysledku
n7:8812098 n7:6990541 n7:4355520
n4:druhVysledku
n10:J
n4:duvernostUdaju
n13:S
n4:entitaPredkladatele
n16:predkladatel
n4:idSjednocenehoVysledku
680084
n4:idVysledku
RIV/68378050:_____/01:00105389
n4:jazykVysledku
n11:eng
n4:klicovaSlova
erythropoietin, mammary gland, transgenic mouse
n4:klicoveSlovo
n6:erythropoietin n6:mammary%20gland n6:transgenic%20mouse
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[7F32C198CC16]
n4:nazevZdroje
Folia Biologica
n4:obor
n8:EB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
6
n4:rokUplatneniVysledku
n17:2001
n4:svazekPeriodika
47
n4:tvurceVysledku
Mikuš, Tomáš Landa, Vladimír Malý, Petr Poplštein, M. Trefil, P. Lidický, J.
n4:zamer
n14:AV0Z5052915
s:issn
0015-5500
s:numberOfPages
9