This HTML5 document contains 55 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n20http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n13http://linked.opendata.cz/resource/domain/vavai/projekt/
n18http://linked.opendata.cz/resource/domain/vavai/subjekt/
n17http://linked.opendata.cz/ontology/domain/vavai/
n4http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n15http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F68378041%3A_____%2F11%3A00368065%21RIV12-AV0-68378041/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n11http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n14http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F68378041%3A_____%2F11%3A00368065%21RIV12-AV0-68378041
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules. Dynamic processes such as diffusion and binding/unbinding of macromolecules (e.g. growth factors or nutrients) are crucial parameters for the design and application of effective artificial tissue materials. Here, dynamics of selected macromolecules were studied in two different composite tissue engineering scaffolds containing an electrospun nanofiber mesh (polycaprolactone or hydrophobically plasma modified polyvinylalcohol–chitosan) encapsulated in agarose hydrogels by a conventional approach fluorescence recovery after photobleaching (FRAP) and a novel technique, raster image correlation spectroscopy (RICS). The two approaches are compared, and it is shown that FRAP is unable to determine processes occurring at low molecular concentrations, especially accurately separating binding/unbinding from diffusion, and its results depend on the concentration of the studied molecules.
dcterms:title
Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds
skos:prefLabel
Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds Raster image correlation spectroscopy as a novel tool to study interactions of macromolecules with nanofiber scaffolds
skos:notation
RIV/68378041:_____/11:00368065!RIV12-AV0-68378041
n17:predkladatel
n18:ico%3A68378041
n3:aktivita
n11:Z n11:P
n3:aktivity
P(GA202/09/1151), P(GAP304/10/1307), P(IAA500390702), P(LC06063), Z(AV0Z40400503), Z(AV0Z50390512), Z(AV0Z50390703), Z(AV0Z50520514)
n3:cisloPeriodika
12
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n20:3458830 Buzgo, Matej
n3:druhVysledku
n12:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
225727
n3:idVysledku
RIV/68378041:_____/11:00368065
n3:jazykVysledku
n16:eng
n3:klicovaSlova
fluorecence dynamics; transient binding; diffusion
n3:klicoveSlovo
n9:fluorecence%20dynamics n9:transient%20binding n9:diffusion
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[50FF43BA0143]
n3:nazevZdroje
Acta Biomaterialia
n3:obor
n5:CF
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
8
n3:projekt
n13:GAP304%2F10%2F1307 n13:GA202%2F09%2F1151 n13:IAA500390702 n13:LC06063
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
7
n3:tvurceVysledku
Buzgo, Matej Lukáš, D. Amler, Evžen Huranová, Martina Humpolíčková, Jana Macháň, Radek Hof, Martin Norris, S. C. P.
n3:wos
000297436500012
n3:zamer
n4:AV0Z40400503 n4:AV0Z50390703 n4:AV0Z50390512 n4:AV0Z50520514
s:issn
1742-7061
s:numberOfPages
9
n15:doi
10.1016/j.actbio.2011.07.012