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Statements

Subject Item
n2:RIV%2F68378041%3A_____%2F11%3A00363241%21RIV12-AV0-68378041
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption. Iron oxide nanoparticles obtained by the coprecipitation of Fe(II) and Fe(III) salts and oxidation were coated with a novel poly(vinyl acetate-co-5-tert-(butylperoxy)-5-methylhex-1-en-3-yne-co-butyl acrylate-co-maleic anhydride) (PVBM) oligomer to ensure colloidal stability. The magnetic nanoparticles were thoroughly characterized by a range of physico-chemical methods, which proved the presence of the coating on the particles. Experiments with rat mesenchymal stem cells (rMSCs) confirmed that PVBM-coated γ-Fe2O3 nanoparticles were not cytotoxic and that the average efficiency of stem cell labeling was good and comparable to that obtained with commercial agents. The cells labeled with PVBM-coated γ-Fe2O3 nanoparticles displayed excellent contrast on magnetic resonance (MR) images. Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers possibility of additional modification by grafting compounds that reduce non-specific protein adsorption.
dcterms:title
The use of oligoperoxide-coated magnetic nanoparticles to label stem cells The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
skos:prefLabel
The use of oligoperoxide-coated magnetic nanoparticles to label stem cells The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
skos:notation
RIV/68378041:_____/11:00363241!RIV12-AV0-68378041
n14:predkladatel
n19:ico%3A68378041
n5:aktivita
n10:P n10:I n10:Z
n5:aktivity
I, P(1M0538), P(GA203/09/1242), P(GAP503/10/0664), P(KAN201110651), P(KAN401220801), Z(AV0Z40500505), Z(AV0Z50390703)
n5:cisloPeriodika
3
n5:dodaniDat
n9:2012
n5:domaciTvurceVysledku
n16:6738109 n16:4798201 n16:1894803
n5:druhVysledku
n12:J
n5:duvernostUdaju
n17:S
n5:entitaPredkladatele
n18:predkladatel
n5:idSjednocenehoVysledku
237244
n5:idVysledku
RIV/68378041:_____/11:00363241
n5:jazykVysledku
n20:eng
n5:klicovaSlova
magnetic; nanoparticles; stem cells
n5:klicoveSlovo
n13:magnetic n13:stem%20cells n13:nanoparticles
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[534E1E95A4BB]
n5:nazevZdroje
Journal of Biomedical Nanotechnology
n5:obor
n8:FH
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
10
n5:projekt
n7:KAN401220801 n7:1M0538 n7:GA203%2F09%2F1242 n7:KAN201110651 n7:GAP503%2F10%2F0664
n5:rokUplatneniVysledku
n9:2011
n5:svazekPeriodika
7
n5:tvurceVysledku
Lesný, Petr Šponarová, Daniela Mitina, N. Stoika, R. Horák, Daniel Jendelová, Pavla Herynek, V. Syková, Eva Trchová, Miroslava Zaichenko, A.
n5:wos
000292858900008
n5:zamer
n15:AV0Z40500505 n15:AV0Z50390703
s:issn
1550-7033
s:numberOfPages
11
n3:doi
10.1166/jbn.2011.1289