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Statements

Subject Item
n2:RIV%2F68081766%3A_____%2F14%3A00424819%21RIV15-GA0-68081766
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 104 tachyzoites. Real-time PCR revealed that even 1–5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys. Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 104 tachyzoites. Real-time PCR revealed that even 1–5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys.
dcterms:title
Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice
skos:prefLabel
Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice Efficacy of magnetic capture in comparison with conventional DNA isolation in a survey of Toxoplasma gondii in wild house mice
skos:notation
RIV/68081766:_____/14:00424819!RIV15-GA0-68081766
n3:aktivita
n6:I n6:S n6:P
n3:aktivity
I, P(ED1.1.00/02.0068), P(GA206/08/0640), S
n3:cisloPeriodika
1
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n17:5592364
n3:druhVysledku
n18:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
13819
n3:idVysledku
RIV/68081766:_____/14:00424819
n3:jazykVysledku
n16:eng
n3:klicovaSlova
House mice; Magnetic capture; Quantitative PCR; Toxoplasma gondii
n3:klicoveSlovo
n4:Quantitative%20PCR n4:Toxoplasma%20gondii n4:House%20mice n4:Magnetic%20capture
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[77ED594A601A]
n3:nazevZdroje
European Journal of Protistology
n3:obor
n13:EG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n8:GA206%2F08%2F0640 n8:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n10:2014
n3:svazekPeriodika
50
n3:tvurceVysledku
Piálek, Jaroslav Hůrková-Hofmannová, L. Baláž, V. Juránková, J. Volf, J.
n3:wos
000331682300002
s:issn
0932-4739
s:numberOfPages
5
n12:doi
10.1016/j.ejop.2013.08.002