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Statements

Subject Item
n2:RIV%2F68081766%3A_____%2F13%3A00396786%21RIV14-AV0-68081766
rdf:type
skos:Concept n6:Vysledek
dcterms:description
The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV. The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV.
dcterms:title
Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
skos:prefLabel
Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
skos:notation
RIV/68081766:_____/13:00396786!RIV14-AV0-68081766
n6:predkladatel
n7:ico%3A68081766
n3:aktivita
n15:I
n3:aktivity
I
n3:cisloPeriodika
40
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n16:9420681
n3:druhVysledku
n14:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
62972
n3:idVysledku
RIV/68081766:_____/13:00396786
n3:jazykVysledku
n10:eng
n3:klicovaSlova
Evolution; Zoonosis; Virome; Metagenomics; Reverse genetics
n3:klicoveSlovo
n5:Virome n5:Evolution n5:Metagenomics n5:Zoonosis n5:Reverse%20genetics
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[39338CB0DFAD]
n3:nazevZdroje
Proceedings of the National Academy of Sciences of the United States of America
n3:obor
n12:EG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
31
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
110
n3:tvurceVysledku
Drosten, C. Annan, A. Vallo, Peter Glebe, D. Geyer, J. Gloza-Rausch, F. Adam, A. Borges Carneiro, A. J. Cottontail, V. M. Van Riel, D. König, A. Ulrich, R. G. Leijten, L. M. Franke, C. R. Leroy, E. Tschapka, M. Bremer, C. M. Binger, T. Oppong, S. Kuiken, T. Gerlich, W. H. Stöcker, A. Geipel, A. Rasche, A. Corman, V. M. Adu-Sarkodie, Y. Schlegel, M. Drexler, J. F. Maganga, G. D. Klose, S. M. Müller, M. A.
n3:wos
000325105500072
s:issn
0027-8424
s:numberOfPages
6
n17:doi
10.1073/pnas.1308049110