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Statements

Subject Item
n2:RIV%2F68081766%3A_____%2F03%3A67030031%21RIV%2F2004%2FAV0%2FA67004%2FN
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 101 plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 10(7) PFU at 7,15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serol Six tests for the detection of West Nile virus (WNV) antibodies in the serum of experimentally infected chickens were compared. The tests included the hemagglutination-inhibition test (HIT), immunoglobulin M (IgM)-capture enzyme-linked immunosorbent assay (ELISA) with WNV-infected mouse brain antigen, immunoglobulin G (IgG) indirect ELISA with tickborne encephalitis viral antigen, the microtitre virus neutralization test, the standard plaque reduction neutralization test (PRNT), and the microtitre PRNT (micro-PRNT). Thirty adult chickens, intravenously and intramuscularly inoculated with 101 plaque-forming units (PFU) of WNV strain Egypt 101, were bled and given a booster of 10(7) PFU at 7,15, and 21 d postinoculation; the final blood collection was on day 28. Although the micro-PRNT is capable of detecting the highest antibody titres during both early and late infection, because of the technical complexity and time requirements of this test a combination of IgM and IgG ELISAs is recommended for serol
dcterms:title
Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Comparison of assays for the detection of West Nile virus antibodies in chicken serum.
skos:prefLabel
Comparison of assays for the detection of West Nile virus antibodies in chicken serum. Comparison of assays for the detection of West Nile virus antibodies in chicken serum.
skos:notation
RIV/68081766:_____/03:67030031!RIV/2004/AV0/A67004/N
n4:strany
128;132
n4:aktivita
n5:Z
n4:aktivity
Z(AV0Z6093917)
n4:cisloPeriodika
2
n4:dodaniDat
n14:2004
n4:domaciTvurceVysledku
n9:2178052 n9:4297873
n4:druhVysledku
n8:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
601683
n4:idVysledku
RIV/68081766:_____/03:67030031
n4:jazykVysledku
n15:eng
n4:klicovaSlova
West Nile virus
n4:klicoveSlovo
n17:West%20Nile%20virus
n4:kodStatuVydavatele
CA - Kanada
n4:kontrolniKodProRIV
[502648ACF61E]
n4:nazevZdroje
Canadian journal of veterinary research
n4:obor
n10:GJ
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
9
n4:pocetUcastnikuAkce
0
n4:pocetZahranicnichUcastnikuAkce
0
n4:rokUplatneniVysledku
n14:2003
n4:svazekPeriodika
67
n4:tvurceVysledku
Andonova, M. Marszal, P. Drebot, M. A. Larence, J. Dibernardo, A. Weingartl, H. M. Halouzka, Jiří Hubálek, Zdeněk Cottam-Birt, C.
n4:zamer
n6:AV0Z6093917
s:issn
0830-9000
s:numberOfPages
5