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Statements

Subject Item
n2:RIV%2F68081731%3A_____%2F09%3A00333759%21RIV10-AV0-68081731
rdf:type
n10:Vysledek skos:Concept
dcterms:description
We present a novel experimental method that solves two key problems in nondestructive mechanical studies of small biomolecules at the single-molecule level, namely the confirmation of single-molecule conditions and the discrimination against nonspecific binding. A biotin avidin ligand-receptor couple is spanned between a glass slide and a 1 mu m latex particles using short linker molecules. Optical tweezers are used to initiate bond formation and to follow the particles thermal position fluctuations with nanometer spatial and microsecond temporal resolution. Here we show that each step in the specific binding process leads to an abrupt change in the magnitude of the particle's thermal position fluctuations, allowing us to count the number of bonds formed one by one. Moreover, three-dimensional position histograms calculated from the particle's fluctuations can be separated into well-defined categories reflecting different binding conditions. We present a novel experimental method that solves two key problems in nondestructive mechanical studies of small biomolecules at the single-molecule level, namely the confirmation of single-molecule conditions and the discrimination against nonspecific binding. A biotin avidin ligand-receptor couple is spanned between a glass slide and a 1 mu m latex particles using short linker molecules. Optical tweezers are used to initiate bond formation and to follow the particles thermal position fluctuations with nanometer spatial and microsecond temporal resolution. Here we show that each step in the specific binding process leads to an abrupt change in the magnitude of the particle's thermal position fluctuations, allowing us to count the number of bonds formed one by one. Moreover, three-dimensional position histograms calculated from the particle's fluctuations can be separated into well-defined categories reflecting different binding conditions.
dcterms:title
Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis
skos:prefLabel
Detecting sequential bond formation using three-dimensional thermal fluctuation analysis Detecting sequential bond formation using three-dimensional thermal fluctuation analysis
skos:notation
RIV/68081731:_____/09:00333759!RIV10-AV0-68081731
n3:aktivita
n5:Z
n3:aktivity
Z(AV0Z20650511)
n3:cisloPeriodika
9-10
n3:dodaniDat
n15:2010
n3:domaciTvurceVysledku
n8:7447213
n3:druhVysledku
n16:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
309762
n3:idVysledku
RIV/68081731:_____/09:00333759
n3:jazykVysledku
n12:eng
n3:klicovaSlova
biophysics; mechanical properties; optical tweezers; single-molecule studies; thermal fluctuations
n3:klicoveSlovo
n7:biophysics n7:optical%20tweezers n7:thermal%20fluctuations n7:single-molecule%20studies n7:mechanical%20properties
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[4847555E2947]
n3:nazevZdroje
ChemPhysChem
n3:obor
n9:BO
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n15:2009
n3:svazekPeriodika
10
n3:tvurceVysledku
Florin, E. L. Jonáš, Alexandr Fisinger, S. Huang, R. Bartsch, T. Kochanczyk, M. D.
n3:wos
000267928100028
n3:zamer
n14:AV0Z20650511
s:issn
1439-4235
s:numberOfPages
7