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Statements

Subject Item
n2:RIV%2F68081715%3A_____%2F14%3A00433692%21RIV15-GA0-68081715
rdf:type
skos:Concept n19:Vysledek
dcterms:description
In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples. In many bioanalytical applications, important molecules such as DNA, proteins, and antibodies are routinely conjugated with fluorescent tags to reach an extraordinary sensitivity of analyses. Semiconductor nanoparticles, quantum dots, have already proved to be suitable components of highly luminescent tags, probes, and sensors with a broad applicability in analytical chemistry. Quantum dots provide high extinction coefficients together with a wide range of excitation wavelengths, size- and composition-tunable emissions, narrow and symmetric emission spectra, good quantum yields, relatively long size-dependent luminescence lifetime, and practically no photobleaching. Most of these properties are superior when compared with conventional organic fluorescent dyes. In this chapter, optimized procedures for the preparation of water-dispersed cadmium telluride (CdTe) quantum dots, conjugating reactions with antibodies, DNA, and macrocycles as well as their analyses by capillary electrophoresis are described. The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples.
dcterms:title
Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
skos:prefLabel
Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
skos:notation
RIV/68081715:_____/14:00433692!RIV15-GA0-68081715
n3:aktivita
n15:P n15:I
n3:aktivity
I, P(GA14-28254S), P(GAP206/11/2377)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n8:6793282 n8:8857741 n8:3926710 n8:6077714
n3:druhVysledku
n18:C
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
2744
n3:idVysledku
RIV/68081715:_____/14:00433692
n3:jazykVysledku
n20:eng
n3:klicovaSlova
capillary electrophoresis; quantum dots; laser-induced fluorescence; immuno assay
n3:klicoveSlovo
n7:immuno%20assay n7:laser-induced%20fluorescence n7:capillary%20electrophoresis n7:quantum%20dots
n3:kontrolniKodProRIV
[BA3632436B2B]
n3:mistoVydani
Totowa
n3:nazevEdiceCisloSvazku
Methods in Molecular Biology, Volume 1199
n3:nazevZdroje
Quantum Dots: Applications in Biology
n3:obor
n17:CB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetStranKnihy
264
n3:pocetTvurcuVysledku
4
n3:projekt
n9:GAP206%2F11%2F2377 n9:GA14-28254S
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Lišková, Marcela Voráčová, Ivona Datinská, Vladimíra Klepárník, Karel
s:numberOfPages
21
n12:doi
10.1007/978-1-4939-1280-3_3
n14:hasPublisher
Humana Press Inc
n5:isbn
978-1-4939-1279-7