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Statements

Subject Item
n2:RIV%2F68081715%3A_____%2F13%3A00420849%21RIV14-MSM-68081715
rdf:type
skos:Concept n5:Vysledek
dcterms:description
Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime. Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
dcterms:title
Senzitive method of Caspase-3 detection in single stem cell Senzitive method of Caspase-3 detection in single stem cell
skos:prefLabel
Senzitive method of Caspase-3 detection in single stem cell Senzitive method of Caspase-3 detection in single stem cell
skos:notation
RIV/68081715:_____/13:00420849!RIV14-MSM-68081715
n5:predkladatel
n6:ico%3A68081715
n3:aktivita
n4:P n4:I
n3:aktivity
I, P(EE2.3.20.0182), P(GAP206/11/2377)
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n18:8857741 n18:1167405
n3:druhVysledku
n8:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
104701
n3:idVysledku
RIV/68081715:_____/13:00420849
n3:jazykVysledku
n14:eng
n3:klicovaSlova
caspase-3; single stem cell; miniaturization
n3:klicoveSlovo
n13:miniaturization n13:single%20stem%20cell n13:caspase-3
n3:kontrolniKodProRIV
[85C6F701EFA0]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Praha
n3:nazevZdroje
Chemické Listy. Roč. 107, Issue s3.
n3:obor
n17:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:projekt
n11:GAP206%2F11%2F2377 n11:EE2.3.20.0182
n3:rokUplatneniVysledku
n7:2013
n3:tvurceVysledku
Matalová, Eva Adamová, Eva Klepárník, Karel
n3:typAkce
n16:WRD
n3:wos
000328730800002
n3:zahajeniAkce
2013-11-12+01:00
s:issn
1213-7103
s:numberOfPages
2
n20:hasPublisher
Česká společnost chemická