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Statements

Subject Item
n2:RIV%2F68081715%3A_____%2F00%3A25200028%21RIV%2F2003%2FAV0%2FA25003%2FN
rdf:type
skos:Concept n18:Vysledek
dcterms:description
Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds. Denaturing capillary electrophoresis provides much faster and more selective separations of DNA fragments than electrophoresis under native conditions. We have developed a highly alkaline electrolyte system with remarkable properties such as extraordinary denaturing power, high separation and low electroosmotic flow mobility in bare fused-silica capillaries. Thus, the analyses of a microsatellite repeat polymorphism in Endothelin 1 gene under denaturing conditions in short capillaries (2.5 cm) with laser-induced fluorescence detection were accomplished in 42 seconds.
dcterms:title
DNA denaturing capillary electrophoresis in highly alkaline solutions. DNA denaturing capillary electrophoresis in highly alkaline solutions.
skos:prefLabel
DNA denaturing capillary electrophoresis in highly alkaline solutions. DNA denaturing capillary electrophoresis in highly alkaline solutions.
skos:notation
RIV/68081715:_____/00:25200028!RIV/2003/AV0/A25003/N
n3:strany
37
n3:aktivita
n17:Z
n3:aktivity
Z(AV0Z4031919)
n3:dodaniDat
n16:2003
n3:domaciTvurceVysledku
n10:8389993 n10:2419858 n10:8857741
n3:druhVysledku
n13:D
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
709005
n3:idVysledku
RIV/68081715:_____/00:25200028
n3:jazykVysledku
n8:eng
n3:klicovaSlova
N/A
n3:klicoveSlovo
n14:N%2FA
n3:kontrolniKodProRIV
[B0EC623472E7]
n3:mistoKonaniAkce
Saarbrücken [DE]
n3:mistoVydani
Saarbrücken
n3:nazevZdroje
13th International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques. Final Program. Book of Abstracts.
n3:obor
n12:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:rokUplatneniVysledku
n16:2000
n3:tvurceVysledku
Boček, Petr Klepárník, Karel Malá, Zdeňka
n3:typAkce
n4:WRD
n3:zahajeniAkce
2000-02-20+01:00
n3:zamer
n15:AV0Z4031919
s:numberOfPages
1
n7:hasPublisher
Neuveden