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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F14%3A00440098%21RIV15-GA0-68081707
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved. Most of the proteins are glycosylated and glycoproteins are involved in physiological and pathological processes. Detection of changes in protein glycosylation is important in early diagnosis of different diseases, including cancer. Among the methods used for the analysis of the carbohydrate components (glycans) of glycoproteins, electrochemical methods were little applied. We propose a modification of glycans directly in glycoproteins using six-valent osmium complexes followed by glycan voltammetric determination at carbon electrodes. The electrochemical responses of two glycoproteins ribonuclease B and avidin as compared to their nonglycosylated counterparts were recorded either directly in the reaction mixture or after a simple purification step. Hundreds of femtomoles of the glycoprotein were sufficient for the analysis. (C) 2014 Elsevier B.V. All rights reserved.
dcterms:title
Direct chemical modification and voltammetric detection of glycans in glycoproteins Direct chemical modification and voltammetric detection of glycans in glycoproteins
skos:prefLabel
Direct chemical modification and voltammetric detection of glycans in glycoproteins Direct chemical modification and voltammetric detection of glycans in glycoproteins
skos:notation
RIV/68081707:_____/14:00440098!RIV15-GA0-68081707
n3:aktivita
n6:I n6:P
n3:aktivity
I, P(ED2.1.00/03.0101), P(GAP301/11/2055)
n3:cisloPeriodika
NOV2014
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n9:9991646 n9:2360551
n3:druhVysledku
n5:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
11636
n3:idVysledku
RIV/68081707:_____/14:00440098
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Glycoproteins; Chemical modification; Os(VI)L complexes
n3:klicoveSlovo
n7:Glycoproteins n7:Os%28VI%29L%20complexes n7:Chemical%20modification
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[D87E1473242C]
n3:nazevZdroje
Electrochemistry Communications
n3:obor
n14:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n8:GAP301%2F11%2F2055 n8:ED2.1.00%2F03.0101
n3:rokUplatneniVysledku
n12:2014
n3:svazekPeriodika
48
n3:tvurceVysledku
Paleček, Emil Trefulka, Mojmír
n3:wos
000345208000014
s:issn
1388-2481
s:numberOfPages
4
n16:doi
10.1016/j.elecom.2014.08.011