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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F12%3A00382270%21RIV13-AV0-68081707
rdf:type
n13:Vysledek skos:Concept
dcterms:description
GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone labels were attached to dCTP through propargyl linker and the resulting labeled nucleotides were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The labeled DNA probes exerted low fluorescence which was increased 2-3.2 times upon binding of a protein. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase. GFP-like 3,5-difluoro-4-hydroxybenzylideneimidazolinone and 3,5-bis(methoxy)-4-hydroxy-benzylideneimidazolinone labels were attached to dCTP through propargyl linker and the resulting labeled nucleotides were used for a facile enzymatic synthesis of oligonucleotide or DNA probes by polymerase-catalyzed primer extension. The labeled DNA probes exerted low fluorescence which was increased 2-3.2 times upon binding of a protein. The FBI label was also used for a time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by Vent(exo-) polymerase.
dcterms:title
GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions
skos:prefLabel
GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions GFP-like Fluorophores as DNA Labels for Studying DNA-Protein Interactions
skos:notation
RIV/68081707:_____/12:00382270!RIV13-AV0-68081707
n13:predkladatel
n18:ico%3A68081707
n3:aktivita
n12:P n12:I
n3:aktivity
I, P(GA203/09/0317), P(GBP206/12/G151)
n3:cisloPeriodika
18
n3:dodaniDat
n14:2013
n3:domaciTvurceVysledku
n9:4171373 n9:2711435
n3:druhVysledku
n6:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
138142
n3:idVysledku
RIV/68081707:_____/12:00382270
n3:jazykVysledku
n4:eng
n3:klicovaSlova
DNA; oligonucleotides; polymerase; nucleotides; fluorescent labeling
n3:klicoveSlovo
n16:nucleotides n16:polymerase n16:DNA n16:fluorescent%20labeling n16:oligonucleotides
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[6EC053D0B686]
n3:nazevZdroje
Journal of Organic Chemistry
n3:obor
n19:CC
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
6
n3:projekt
n10:GBP206%2F12%2FG151 n10:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n14:2012
n3:svazekPeriodika
77
n3:tvurceVysledku
Orság, Petr Riedl, Jan Hocek, Michal Fojta, Miroslav Pohl, Radek Ménová, Petra
n3:wos
000308957700049
s:issn
0022-3263
s:numberOfPages
7
n8:doi
10.1021/jo301684b