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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F12%3A00380149%21RIV13-AV0-68081707
rdf:type
n14:Vysledek skos:Concept
dcterms:description
In an attempt to develop a label-free electrochemical method for detection of changes in protein structures based on oxidizability of tyrosine and tryptophan residues we tested different types of carbon electrodes. We found that using edge plane pyrolytic graphite electrode we can discriminate between native and denatured forms of human serum albumin and of other proteins. The electrochemical denaturation curve agreed reasonably well with that based on intrinsic fluorescence of tyrosine and tryptophan. It can be expected that the electrochemical method will be applicable to a large number of proteins and may become useful in biomedicine and proteomics. In an attempt to develop a label-free electrochemical method for detection of changes in protein structures based on oxidizability of tyrosine and tryptophan residues we tested different types of carbon electrodes. We found that using edge plane pyrolytic graphite electrode we can discriminate between native and denatured forms of human serum albumin and of other proteins. The electrochemical denaturation curve agreed reasonably well with that based on intrinsic fluorescence of tyrosine and tryptophan. It can be expected that the electrochemical method will be applicable to a large number of proteins and may become useful in biomedicine and proteomics.
dcterms:title
Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes
skos:prefLabel
Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes
skos:notation
RIV/68081707:_____/12:00380149!RIV13-AV0-68081707
n14:predkladatel
n17:ico%3A68081707
n3:aktivita
n7:Z n7:P
n3:aktivity
P(GAP301/11/2055), P(KJB100040901), P(ME09038), Z(AV0Z50040702)
n3:cisloPeriodika
JUL
n3:dodaniDat
n6:2013
n3:domaciTvurceVysledku
n5:6281044 n5:9991646 n5:9011390
n3:druhVysledku
n19:J
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
153179
n3:idVysledku
RIV/68081707:_____/12:00380149
n3:jazykVysledku
n15:eng
n3:klicovaSlova
protein denaturation; carbon electrodes; edge plane pyrolytic graphite
n3:klicoveSlovo
n11:edge%20plane%20pyrolytic%20graphite n11:carbon%20electrodes n11:protein%20denaturation
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[9D2D6EB54419]
n3:nazevZdroje
Analytica Chimica Acta
n3:obor
n10:BO
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n8:GAP301%2F11%2F2055 n8:KJB100040901 n8:ME09038
n3:rokUplatneniVysledku
n6:2012
n3:svazekPeriodika
735
n3:tvurceVysledku
Černocká, Hana Paleček, Emil Kurzatkowska, K. Ostatná, Veronika
n3:wos
000306150400004
n3:zamer
n9:AV0Z50040702
s:issn
0003-2670
s:numberOfPages
6
n16:doi
10.1016/j.aca.2012.05.012