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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F11%3A00360998%21RIV12-AV0-68081707
rdf:type
n3:Vysledek skos:Concept
dcterms:description
Aqueous Suzuki-Miyaura cross-coupling reactions of halogenated nucleosides, nucleotides and nucleoside triphosphates (dNTPs) derived from 5-iodocytosine and 7-iodo-7-deazaadenine with methyl-, benzyl- and tritylsufanylphenylboronic acids gave the corresponding alkylsulfanylphenyl derivatives of nucleosides and nucleotides. The modified dNTPs were incorporated to DNA by primer extension using Vent(exo-) polymerase. Electrochemical behaviour of the alkyl-sulfanylphenyl nucleosides indicated formation of compact layers on the electrode. Modified nucleotides and DNA bearing benzyl- or tritylsulfanylphenyl moieties produced signals in [Co(NH3)6]3+ ammonium buffer attributable to the Brdicka catalytic reponses (BCR), depending on negative potential applied. Repeated potential scans in this medium of constant current chronopotentiometric showed increased BCR suggesting the deprotection of the alkylsulfanyl derivatives to free thiols under those conditions. Aqueous Suzuki-Miyaura cross-coupling reactions of halogenated nucleosides, nucleotides and nucleoside triphosphates (dNTPs) derived from 5-iodocytosine and 7-iodo-7-deazaadenine with methyl-, benzyl- and tritylsufanylphenylboronic acids gave the corresponding alkylsulfanylphenyl derivatives of nucleosides and nucleotides. The modified dNTPs were incorporated to DNA by primer extension using Vent(exo-) polymerase. Electrochemical behaviour of the alkyl-sulfanylphenyl nucleosides indicated formation of compact layers on the electrode. Modified nucleotides and DNA bearing benzyl- or tritylsulfanylphenyl moieties produced signals in [Co(NH3)6]3+ ammonium buffer attributable to the Brdicka catalytic reponses (BCR), depending on negative potential applied. Repeated potential scans in this medium of constant current chronopotentiometric showed increased BCR suggesting the deprotection of the alkylsulfanyl derivatives to free thiols under those conditions.
dcterms:title
Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates. Synthesis, polymerase incorporation to DNA and electrochemical study Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates. Synthesis, polymerase incorporation to DNA and electrochemical study
skos:prefLabel
Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates. Synthesis, polymerase incorporation to DNA and electrochemical study Alkylsulfanylphenyl derivatives of cytosine and 7-deazaadenine nucleosides, nucleotides and nucleoside triphosphates. Synthesis, polymerase incorporation to DNA and electrochemical study
skos:notation
RIV/68081707:_____/11:00360998!RIV12-AV0-68081707
n3:predkladatel
n4:ico%3A68081707
n5:aktivita
n20:Z n20:P
n5:aktivity
P(GA203/09/0317), P(IAA400040901), P(LC06035), P(LC512), Z(AV0Z40550506), Z(AV0Z50040507), Z(AV0Z50040702)
n5:cisloPeriodika
21
n5:dodaniDat
n16:2012
n5:domaciTvurceVysledku
n13:6875181 n13:3288641 n13:4171373 n13:4328647
n5:druhVysledku
n8:J
n5:duvernostUdaju
n19:S
n5:entitaPredkladatele
n6:predkladatel
n5:idSjednocenehoVysledku
185217
n5:idVysledku
RIV/68081707:_____/11:00360998
n5:jazykVysledku
n12:eng
n5:klicovaSlova
DNA polymerases; electrochemistry; nucleosides; nucleotides; organosulfur compounds
n5:klicoveSlovo
n10:nucleotides n10:electrochemistry n10:organosulfur%20compounds n10:nucleosides n10:DNA%20polymerases
n5:kodStatuVydavatele
DE - Spolková republika Německo
n5:kontrolniKodProRIV
[9F287B7B8D69]
n5:nazevZdroje
Chemistry - A European Journal
n5:obor
n17:CC
n5:pocetDomacichTvurcuVysledku
4
n5:pocetTvurcuVysledku
7
n5:projekt
n14:GA203%2F09%2F0317 n14:IAA400040901 n14:LC512 n14:LC06035
n5:rokUplatneniVysledku
n16:2011
n5:svazekPeriodika
17
n5:tvurceVysledku
Hocek, Michal Fojta, Miroslav Macíčková-Cahová, Hana Špaček, Jan Pohl, Radek Havran, Luděk Horáková, Petra
n5:wos
000291594800011
n5:zamer
n18:AV0Z50040507 n18:AV0Z40550506 n18:AV0Z50040702
s:issn
0947-6539
s:numberOfPages
9
n9:doi
10.1002/chem.201003496