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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F05%3A00001223%21RIV06-AV0-68081707
rdf:type
n16:Vysledek skos:Concept
dcterms:description
Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed. Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed. Pomocí elektronové mikroskopie byly analyzovány interakce proteinů p53 exprimovaných v bakteriích, celého proteinu p53, p53(1-393) a C-terminálního fragmentu p53(320-393), s dlouhou dsDNA (přibližně 3000 bp) v lineární a superhelikální formě obsahující či neobsahující proteinem p53 rozpoznávanou sekvenci (p53CON). Hlavním strukturním znakem vytvořených komplexů byl DNA -protein filament, ve kterém jsou duplexy DNA spojeny díky navázanému tetrametru proteinu. V práci byly diskutovány možné důsledky zachycení p53 prostřednictvím tvorby těchto DNA-proteinových filament.
dcterms:title
Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou Sequestering of p53 into DNA-protein filaments revealed by electron microscopy Sequestering of p53 into DNA-protein filaments revealed by electron microscopy
skos:prefLabel
Sequestering of p53 into DNA-protein filaments revealed by electron microscopy Sequestering of p53 into DNA-protein filaments revealed by electron microscopy Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou
skos:notation
RIV/68081707:_____/05:00001223!RIV06-AV0-68081707
n3:strany
261;271
n3:aktivita
n7:P n7:Z
n3:aktivity
P(GA301/02/0831), P(GA301/99/D078), P(IAA4004110), P(IAA500040513), Z(AV0Z50040507)
n3:cisloPeriodika
2-3
n3:dodaniDat
n9:2006
n3:domaciTvurceVysledku
n4:9991646 n4:6920454 n4:8312095
n3:druhVysledku
n18:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
542394
n3:idVysledku
RIV/68081707:_____/05:00001223
n3:jazykVysledku
n17:eng
n3:klicovaSlova
p53; electron microscopy; DNA-protein complex
n3:klicoveSlovo
n6:p53 n6:DNA-protein%20complex n6:electron%20microscopy
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[DAD38E2973DF]
n3:nazevZdroje
Biophysical Chemistry
n3:obor
n14:BO
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n5:GA301%2F99%2FD078 n5:IAA500040513 n5:GA301%2F02%2F0831 n5:IAA4004110
n3:rokUplatneniVysledku
n9:2005
n3:svazekPeriodika
14
n3:tvurceVysledku
Jovin, T. M. Paleček, Emil Cherny, Dmitry I. Paleček, Jan Brázdová, Marie
n3:zamer
n11:AV0Z50040507
s:issn
0301-4622
s:numberOfPages
11