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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F03%3A17033126%21RIV%2F2004%2FAV0%2FA17004%2FN
rdf:type
skos:Concept n18:Vysledek
dcterms:description
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields. The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quatifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
dcterms:title
Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize.
skos:prefLabel
Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize. Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize.
skos:notation
RIV/68081707:_____/03:17033126!RIV/2004/AV0/A17004/N
n3:strany
2663;2669
n3:aktivita
n16:P n16:Z
n3:aktivity
P(GA203/02/0422), P(IAA1163201), P(IBS5004107), Z(AV0Z5004920)
n3:cisloPeriodika
11
n3:dodaniDat
n10:2004
n3:domaciTvurceVysledku
n8:4995775 n8:3931315 n8:4637844 n8:9629378 n8:8312095
n3:druhVysledku
n14:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
598834
n3:idVysledku
RIV/68081707:_____/03:17033126
n3:jazykVysledku
n11:eng
n3:klicovaSlova
carbon electrodes; mercury electrodes; streptavidin
n3:klicoveSlovo
n13:mercury%20electrodes n13:carbon%20electrodes n13:streptavidin
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[FF9485BD14A1]
n3:nazevZdroje
Analytical Chemistry
n3:obor
n15:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
7
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n9:GA203%2F02%2F0422 n9:IBS5004107 n9:IAA1163201
n3:rokUplatneniVysledku
n10:2003
n3:svazekPeriodika
75
n3:tvurceVysledku
Kizek, René Jelen, František Howard, J. A. Kramer, K. J. Masařík, Michal Billová, Sabina Brázdová, Marie
n3:zamer
n12:AV0Z5004920
s:issn
0003-2700
s:numberOfPages
7