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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F03%3A17033047%21RIV%2F2004%2FAV0%2FA17004%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
We are exploring the design of platinum complexes with low toxicity that could be photoactivated selectively at the target site. We show here that the Pt(IV) azide complex cis,trans-[Pt(en)(N3)2(OH)2] is unreactive towards DNA until irradiated with visible light. Transcription mapping studies of DNA treated with cis,trans-[Pt(en)(N3)2(OH)2] and irradiated with visible light showed that the platination sites were similar to those observed for cisplatin, and were mainly in GG sequences. HPLC analysis of enzymatic digests of an irradiated sample of DNA duplex treated with the same complex also revealed preferential formation of GG cross-links. Since such DNA lesions are thought to be responsible for the induction of apoptosis in cancer cells by platinum drugs, the use of unreactive photoactivatable platinum pro-drugs may become an effective strategy for the design of a new generation of platinum anticancer complexes. We are exploring the design of platinum complexes with low toxicity that could be photoactivated selectively at the target site. We show here that the Pt(IV) azide complex cis,trans-[Pt(en)(N3)2(OH)2] is unreactive towards DNA until irradiated with visible light. Transcription mapping studies of DNA treated with cis,trans-[Pt(en)(N3)2(OH)2] and irradiated with visible light showed that the platination sites were similar to those observed for cisplatin, and were mainly in GG sequences. HPLC analysis of enzymatic digests of an irradiated sample of DNA duplex treated with the same complex also revealed preferential formation of GG cross-links. Since such DNA lesions are thought to be responsible for the induction of apoptosis in cancer cells by platinum drugs, the use of unreactive photoactivatable platinum pro-drugs may become an effective strategy for the design of a new generation of platinum anticancer complexes.
dcterms:title
Formation of platinated GG cross-links on DNA by photoactivation of a Pt(IV) azide complex. Formation of platinated GG cross-links on DNA by photoactivation of a Pt(IV) azide complex.
skos:prefLabel
Formation of platinated GG cross-links on DNA by photoactivation of a Pt(IV) azide complex. Formation of platinated GG cross-links on DNA by photoactivation of a Pt(IV) azide complex.
skos:notation
RIV/68081707:_____/03:17033047!RIV/2004/AV0/A17004/N
n3:strany
741;745
n3:aktivita
n5:P n5:Z
n3:aktivity
P(IAA5004101), P(KJB5004301), Z(AV0Z5004920)
n3:cisloPeriodika
7
n3:dodaniDat
n7:2004
n3:domaciTvurceVysledku
n9:4571932 n9:7988346
n3:druhVysledku
n4:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
607587
n3:idVysledku
RIV/68081707:_____/03:17033047
n3:jazykVysledku
n12:eng
n3:klicovaSlova
cross-links; DNA; guanine
n3:klicoveSlovo
n11:cross-links n11:guanine n11:DNA
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2974D4B73AC7]
n3:nazevZdroje
Journal of Biological Inorganic Chemistry
n3:obor
n18:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n10:KJB5004301 n10:IAA5004101
n3:rokUplatneniVysledku
n7:2003
n3:svazekPeriodika
8
n3:tvurceVysledku
Mackay, F. S. Sadler, P. J. Kašpárková, Jana Brabec, Viktor
n3:zamer
n13:AV0Z5004920
s:issn
0949-8257
s:numberOfPages
5