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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F02%3A17023186%21RIV%2F2003%2FGA0%2FA17003%2FN
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants. Electroporation represents a powerful technique for cell transfection; however, its efficiency in haemopoietic cells (~1%) is largely unsatisfactory. Biological processes in haemopoietic cells are often studied using leukemia cell line HL-60. For this reason we developed conditions for efficiently introducing plasmids to HL-60 cells by electroporation, as an alternative to other techniques. This technique employs electric pulse (250-270 V; 1000 microF) followed by separation of living cells on Ficoll-Paque discontinuous gradient. Using 10-20 microg of plasmid, we routinely achieve 12-14% of transfectants.
dcterms:title
Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.
skos:prefLabel
Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation. Conditions for gene transfection into the HL-60 human leukaemia cell line by electroporation.
skos:notation
RIV/68081707:_____/02:17023186!RIV/2003/GA0/A17003/N
n3:strany
154;156
n3:aktivita
n6:P n6:Z
n3:aktivity
P(GA524/99/0694), P(IBS5004009), P(KSK5011112), Z(AV0Z5004920)
n3:cisloPeriodika
4
n3:dodaniDat
n7:2003
n3:domaciTvurceVysledku
n5:1706063 n5:1075020 n5:8855803 n5:6215750 n5:3757897
n3:druhVysledku
n16:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
641562
n3:idVysledku
RIV/68081707:_____/02:17023186
n3:jazykVysledku
n12:eng
n3:klicovaSlova
leukaemia cell line HL-60; electroporation; gene transfection
n3:klicoveSlovo
n10:electroporation n10:gene%20transfection n10:leukaemia%20cell%20line%20HL-60
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[E6F9EDD4D9AC]
n3:nazevZdroje
Folia Biologica
n3:obor
n4:BO
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n9:GA524%2F99%2F0694 n9:IBS5004009 n9:KSK5011112
n3:rokUplatneniVysledku
n7:2002
n3:svazekPeriodika
48
n3:tvurceVysledku
Kozubík, Alois Janík, Robert Pacherník, Jiří Hofmanová, Jiřina Bryja, Vítězslav
n3:zamer
n18:AV0Z5004920
s:issn
0015-5500
s:numberOfPages
3