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Statements

Subject Item
n2:RIV%2F68081707%3A_____%2F02%3A17023049%21RIV%2F2003%2FGA0%2FA17003%2FN
rdf:type
n13:Vysledek skos:Concept
dcterms:description
Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample. Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample.
dcterms:title
Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393).
skos:prefLabel
Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393).
skos:notation
RIV/68081707:_____/02:17023049!RIV/2003/GA0/A17003/N
n3:strany
115;118
n3:aktivita
n9:P n9:Z
n3:aktivity
P(GA204/00/D049), P(GV204/97/K084), P(IAA4004110), P(NC5343), Z(AV0Z5004920)
n3:cisloPeriodika
1/2
n3:dodaniDat
n11:2003
n3:domaciTvurceVysledku
n10:4995775 n10:3288641 n10:8312095 n10:9991646
n3:druhVysledku
n8:J
n3:duvernostUdaju
n14:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
642721
n3:idVysledku
RIV/68081707:_____/02:17023049
n3:jazykVysledku
n4:eng
n3:klicovaSlova
p53; glutathione-S-transferase determination; constant current chronopotentiometry
n3:klicoveSlovo
n5:glutathione-S-transferase%20determination n5:p53 n5:constant%20current%20chronopotentiometry
n3:kodStatuVydavatele
CH - Švýcarská konfederace
n3:kontrolniKodProRIV
[83292A39FEAB]
n3:nazevZdroje
Bioelectrochemistry
n3:obor
n17:BO
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:pocetUcastnikuAkce
0
n3:pocetZahranicnichUcastnikuAkce
0
n3:projekt
n7:IAA4004110 n7:GA204%2F00%2FD049 n7:NC5343 n7:GV204%2F97%2FK084
n3:rokUplatneniVysledku
n11:2002
n3:svazekPeriodika
55
n3:tvurceVysledku
Havran, Luděk Kizek, René Paleček, Emil Brázdová, Marie
n3:zamer
n16:AV0Z5004920
s:issn
1567-5394
s:numberOfPages
4