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Statements

Subject Item
n2:RIV%2F67985939%3A_____%2F14%3A00436267%21RIV15-GA0-67985939
rdf:type
n11:Vysledek skos:Concept
dcterms:description
With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatment of the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described. With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status and many other aspects of plant biology. Enormous herbarium collections are an important source of material especially for specimens from areas difficult to access or from taxa that are now extinct. The ability to utilize these specimens greatly enhances the research. However the process of extracting DNA from herbarium specimens is often fraught with difficulty related to such variables as plant chemistry, drying method of the specimen and chemical treatment of the specimen. Although many methods have been developed for extraction of DNA from herbarium specimens, the most frequently used are modified CTAB and DNeasy Plant Mini Kit protocols. Nine selected protocols have been described.
dcterms:title
DNA extraction from herbarium specimens DNA extraction from herbarium specimens
skos:prefLabel
DNA extraction from herbarium specimens DNA extraction from herbarium specimens
skos:notation
RIV/67985939:_____/14:00436267!RIV15-GA0-67985939
n3:aktivita
n4:I n4:P
n3:aktivity
I, P(GAP506/11/0774), P(GP206/07/P147)
n3:dodaniDat
n16:2015
n3:domaciTvurceVysledku
n5:8727570
n3:druhVysledku
n14:C
n3:duvernostUdaju
n20:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
11996
n3:idVysledku
RIV/67985939:_____/14:00436267
n3:jazykVysledku
n12:eng
n3:klicovaSlova
DNA; herbarium speciemen; plants
n3:klicoveSlovo
n19:herbarium%20speciemen n19:plants n19:DNA
n3:kontrolniKodProRIV
[E9ED8B67D272]
n3:mistoVydani
New York
n3:nazevEdiceCisloSvazku
Methods in Molecular Biology
n3:nazevZdroje
Molecular Plant Taxonomy: Methods and protocols
n3:obor
n10:EF
n3:pocetDomacichTvurcuVysledku
1
n3:pocetStranKnihy
402
n3:pocetTvurcuVysledku
1
n3:projekt
n13:GAP506%2F11%2F0774 n13:GP206%2F07%2FP147
n3:rokUplatneniVysledku
n16:2014
n3:tvurceVysledku
Záveská Drábková, Lenka
s:numberOfPages
18
n8:doi
10.1007/978-1-62703-767-9_4
n7:hasPublisher
Springer Science+Business Media
n17:isbn
978-1-62703-766-2